The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae

Volodymyr Radchuk (Lead / Corresponding author), Rajiv Sharma, Elena Potokina, Ruslana Radchuk, Diana Weier, Eberhard Munz, Miriam Schreiber, Martin Mascher, Nils Stein, Thomas Wicker, Benjamin Kilian, Ljudmilla Borisjuk

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Abstract

Phylogenetically related groups of species contain lineage-specific genes that exhibit no sequence similarity to any genes outside the lineage. We describe here that the Jekyll gene, required for sexual reproduction, exists in two much diverged allelic variants, Jek1 and Jek3. Despite low similarity, the Jek1 and Jek3 proteins share identical signal peptides, conserved cysteine positions and direct repeats. The Jek1/Jek3 sequences are located at the same chromosomal locus and inherited in a monogenic Mendelian fashion. Jek3 has a similar expression as Jek1 and complements the Jek1 function in Jek1-deficient plants. Jek1 and Jek3 allelic variants were almost equally distributed in a collection of 485 wild and domesticated barley accessions. All domesticated barleys harboring the Jek1 allele belong to single haplotype J1-H1 indicating a genetic bottleneck during domestication. Domesticated barleys harboring the Jek3 allele consisted of three haplotypes. Jekyll-like sequences were found only in species of the closely related tribes Bromeae and Triticeae but not in other Poaceae. Non-invasive magnetic resonance imaging revealed intrinsic grain structure in Triticeae and Bromeae, associated with the Jekyll function. The emergence of Jekyll suggests its role in the separation of the Bromeae and Triticeae lineages within the Poaceae and identifies the Jekyll genes as lineage-specific.

Original languageEnglish
Pages (from-to)961-974
Number of pages14
JournalPlant Journal
Volume98
Issue number6
Early online date25 Apr 2019
DOIs
Publication statusPublished - Jun 2019

Fingerprint

Poaceae
sexual reproduction
Reproduction
Hordeum
grasses
barley
Haplotypes
Genes
haplotypes
genes
Alleles
alleles
Nucleic Acid Repetitive Sequences
Protein Sorting Signals
signal peptide
domestication
magnetic resonance imaging
Cysteine
cysteine
complement

Keywords

  • Hordeum vulgare
  • Triticeae
  • Triticum aestivum
  • gene alleles
  • gene family evolution
  • lineage-specific genes
  • plant reproduction

Cite this

Radchuk, Volodymyr ; Sharma, Rajiv ; Potokina, Elena ; Radchuk, Ruslana ; Weier, Diana ; Munz, Eberhard ; Schreiber, Miriam ; Mascher, Martin ; Stein, Nils ; Wicker, Thomas ; Kilian, Benjamin ; Borisjuk, Ljudmilla. / The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae. In: Plant Journal. 2019 ; Vol. 98, No. 6. pp. 961-974.
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abstract = "Phylogenetically related groups of species contain lineage-specific genes that exhibit no sequence similarity to any genes outside the lineage. We describe here that the Jekyll gene, required for sexual reproduction, exists in two much diverged allelic variants, Jek1 and Jek3. Despite low similarity, the Jek1 and Jek3 proteins share identical signal peptides, conserved cysteine positions and direct repeats. The Jek1/Jek3 sequences are located at the same chromosomal locus and inherited in a monogenic Mendelian fashion. Jek3 has a similar expression as Jek1 and complements the Jek1 function in Jek1-deficient plants. Jek1 and Jek3 allelic variants were almost equally distributed in a collection of 485 wild and domesticated barley accessions. All domesticated barleys harboring the Jek1 allele belong to single haplotype J1-H1 indicating a genetic bottleneck during domestication. Domesticated barleys harboring the Jek3 allele consisted of three haplotypes. Jekyll-like sequences were found only in species of the closely related tribes Bromeae and Triticeae but not in other Poaceae. Non-invasive magnetic resonance imaging revealed intrinsic grain structure in Triticeae and Bromeae, associated with the Jekyll function. The emergence of Jekyll suggests its role in the separation of the Bromeae and Triticeae lineages within the Poaceae and identifies the Jekyll genes as lineage-specific.",
keywords = "Hordeum vulgare, Triticeae, Triticum aestivum, gene alleles, gene family evolution, lineage-specific genes, plant reproduction",
author = "Volodymyr Radchuk and Rajiv Sharma and Elena Potokina and Ruslana Radchuk and Diana Weier and Eberhard Munz and Miriam Schreiber and Martin Mascher and Nils Stein and Thomas Wicker and Benjamin Kilian and Ljudmilla Borisjuk",
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Radchuk, V, Sharma, R, Potokina, E, Radchuk, R, Weier, D, Munz, E, Schreiber, M, Mascher, M, Stein, N, Wicker, T, Kilian, B & Borisjuk, L 2019, 'The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae', Plant Journal, vol. 98, no. 6, pp. 961-974. https://doi.org/10.1111/tpj.14363

The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae. / Radchuk, Volodymyr (Lead / Corresponding author); Sharma, Rajiv; Potokina, Elena; Radchuk, Ruslana; Weier, Diana; Munz, Eberhard; Schreiber, Miriam; Mascher, Martin; Stein, Nils; Wicker, Thomas; Kilian, Benjamin; Borisjuk, Ljudmilla.

In: Plant Journal, Vol. 98, No. 6, 06.2019, p. 961-974.

Research output: Contribution to journalArticle

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T1 - The highly divergent Jekyll genes, required for sexual reproduction, are lineage specific for the related grass tribes Triticeae and Bromeae

AU - Radchuk, Volodymyr

AU - Sharma, Rajiv

AU - Potokina, Elena

AU - Radchuk, Ruslana

AU - Weier, Diana

AU - Munz, Eberhard

AU - Schreiber, Miriam

AU - Mascher, Martin

AU - Stein, Nils

AU - Wicker, Thomas

AU - Kilian, Benjamin

AU - Borisjuk, Ljudmilla

N1 - © 2019 The Authors The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.

PY - 2019/6

Y1 - 2019/6

N2 - Phylogenetically related groups of species contain lineage-specific genes that exhibit no sequence similarity to any genes outside the lineage. We describe here that the Jekyll gene, required for sexual reproduction, exists in two much diverged allelic variants, Jek1 and Jek3. Despite low similarity, the Jek1 and Jek3 proteins share identical signal peptides, conserved cysteine positions and direct repeats. The Jek1/Jek3 sequences are located at the same chromosomal locus and inherited in a monogenic Mendelian fashion. Jek3 has a similar expression as Jek1 and complements the Jek1 function in Jek1-deficient plants. Jek1 and Jek3 allelic variants were almost equally distributed in a collection of 485 wild and domesticated barley accessions. All domesticated barleys harboring the Jek1 allele belong to single haplotype J1-H1 indicating a genetic bottleneck during domestication. Domesticated barleys harboring the Jek3 allele consisted of three haplotypes. Jekyll-like sequences were found only in species of the closely related tribes Bromeae and Triticeae but not in other Poaceae. Non-invasive magnetic resonance imaging revealed intrinsic grain structure in Triticeae and Bromeae, associated with the Jekyll function. The emergence of Jekyll suggests its role in the separation of the Bromeae and Triticeae lineages within the Poaceae and identifies the Jekyll genes as lineage-specific.

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KW - Hordeum vulgare

KW - Triticeae

KW - Triticum aestivum

KW - gene alleles

KW - gene family evolution

KW - lineage-specific genes

KW - plant reproduction

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