TY - JOUR
T1 - The human T3 γ chain is phosphorylated at serine 126 in response to T lymphocyte activation
AU - Davies, A. A.
AU - Cantrell, D. A.
AU - Hexham, J. M.
AU - Parker, P. J.
AU - Rothbard, J.
AU - Crumpton, M. J.
PY - 1987/8/15
Y1 - 1987/8/15
N2 - The γ subunit of the human T lymphocyte T3 antigen is rapidly phosphorylated on serine residues in vivo during the initiation of T cell activation by a polyclonal mitogen (Phaseolus vulgaris phytohemagglutinin), an activator of protein kinase C (phorbol 12,13-dibutyrate), and an elevator of intracellular calcium (ionomycin). The sites of phosphorylation were identified by comparing tryptic peptide analyses of T3 γ chains labeled in vivo with various synthetic peptides, corresponding to portions of the cytoplasmic domain of the γ chain that had been labeled in vitro using purified protein kinase C. Two sites, serines 123 and 126, were phosphorylated in response to ionomycin, whereas a single site, serine 126, was phosphorylated when T lymphocytes were stimulated by P. vulgaris phytohemagglutinin or when protein kinase C was directly activated by phorbol 12,13-dibutyrate. Immune activation of T cells via the protein kinase C pathway thus induces phosphorylation of a single site on the T3 γ chain, namely serine 126.
AB - The γ subunit of the human T lymphocyte T3 antigen is rapidly phosphorylated on serine residues in vivo during the initiation of T cell activation by a polyclonal mitogen (Phaseolus vulgaris phytohemagglutinin), an activator of protein kinase C (phorbol 12,13-dibutyrate), and an elevator of intracellular calcium (ionomycin). The sites of phosphorylation were identified by comparing tryptic peptide analyses of T3 γ chains labeled in vivo with various synthetic peptides, corresponding to portions of the cytoplasmic domain of the γ chain that had been labeled in vitro using purified protein kinase C. Two sites, serines 123 and 126, were phosphorylated in response to ionomycin, whereas a single site, serine 126, was phosphorylated when T lymphocytes were stimulated by P. vulgaris phytohemagglutinin or when protein kinase C was directly activated by phorbol 12,13-dibutyrate. Immune activation of T cells via the protein kinase C pathway thus induces phosphorylation of a single site on the T3 γ chain, namely serine 126.
UR - http://www.scopus.com/inward/record.url?scp=0023212635&partnerID=8YFLogxK
M3 - Article
C2 - 3112151
AN - SCOPUS:0023212635
VL - 262
SP - 10918
EP - 10921
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 23
ER -