The Hybrid Type Polyketide Synthase SteelyA Is Required for cAMP Signalling in Early Dictyostelium Development

Takaaki B. Narita, Zhi-Hui Chen, Pauline Schaap, Tamao Saito (Lead / Corresponding author)

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    11 Citations (Scopus)

    Abstract

    Background

    In our previous study we found that the expression of stlA showed peaks both in the early and last stages of development and that a product of SteelyA, 4-methyl-5-pentylbenzene-1,3-diol (MPBD), controlled Dictyostelium spore maturation during the latter. In this study we focused on the role of SteelyA in early stage development.
    Principal Findings

    Our stlA null mutant showed aggregation delay and abnormally small aggregation territories. Chemotaxis analysis revealed defective cAMP chemotaxis in the stlA null mutant. cAMP chemotaxis was restored by MPBD addition during early stage development. Assay for cAMP relay response revealed that the stlA null mutant had lower cAMP accumulation during aggregation, suggesting lower ACA activity than the wild type strain. Exogenous cAMP pulses rescued the aggregation defect of the stlA null strain in the absence of MPBD. Expression analysis of cAMP signalling genes revealed lower expression levels in the stlA null mutant during aggregation.
    Conclusion

    Our data indicate a regulatory function by SteelyA on cAMP signalling during aggregation and show that SteelyA is indispensable for full activation of ACA.
    Original languageEnglish
    Article numbere106634
    Number of pages9
    JournalPLoS ONE
    Volume9
    Issue number9
    DOIs
    Publication statusPublished - 15 Sep 2014

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