The hydrophobic mannoside Manα1-6Manα1-S-(CH2)7-CH3 acts as an acceptor for the UDP-Gal: glycosylphosphatidylinositol anchor α1,3-galactosyltransferase of Trypanosoma brucei

TY - JOUR

T1 - The hydrophobic mannoside Manα1-6Manα1-S-(CH2)7-CH3 acts as an acceptor for the UDP-Gal

T2 - glycosylphosphatidylinositol anchor α1,3-galactosyltransferase of Trypanosoma brucei

AU - Pingel, Sabine

AU - Field, Robert A.

AU - Guther, Maria Lucia S.

AU - Duszenko, Michael

AU - Ferguson, Michael A. J.

N1 - Medline is the source for the MeSH terms of this document.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The variant surface glycoproteins (VSGs) of Trypanosoma brucei are attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) membrane anchor, This anchor contains the core sequence ethanolamine-PO4-6Mana1-2Mana1-6Mana1-4GlcNa1-6myo- inositol, which is conserved in all GPI anchors, and a unique aGal side chain attached to the 3-position of the aMan residue adjacent to the aGlcN residue. Here we report that trypanosome membranes can catalyse the transfer of Gal from UDP-Gal to the hydrophobic thioglycoside Mana1-6Mana1-S-(CH2)7-CH2. Characterization of the galactosylated products by electrospray mass spectrometry, exoglycosidase digestion and periodate-oxidation studies revealed that the major product was Mana1-6(Gala1-3)Mana1-S-(CH2)7-CH2. The similarity of this product to part of the mature VSG GPI anchor suggests that the thioglycoside is able to act as an acceptor for the trypanosome-specific UDP-Gal-GPI anchor a1,3-galactosyltransferase.

AB - The variant surface glycoproteins (VSGs) of Trypanosoma brucei are attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) membrane anchor, This anchor contains the core sequence ethanolamine-PO4-6Mana1-2Mana1-6Mana1-4GlcNa1-6myo- inositol, which is conserved in all GPI anchors, and a unique aGal side chain attached to the 3-position of the aMan residue adjacent to the aGlcN residue. Here we report that trypanosome membranes can catalyse the transfer of Gal from UDP-Gal to the hydrophobic thioglycoside Mana1-6Mana1-S-(CH2)7-CH2. Characterization of the galactosylated products by electrospray mass spectrometry, exoglycosidase digestion and periodate-oxidation studies revealed that the major product was Mana1-6(Gala1-3)Mana1-S-(CH2)7-CH2. The similarity of this product to part of the mature VSG GPI anchor suggests that the thioglycoside is able to act as an acceptor for the trypanosome-specific UDP-Gal-GPI anchor a1,3-galactosyltransferase.

UR - https://www.scopus.com/pages/publications/0028980977

M3 - Article

SN - 0264-6021

VL - 309

SP - 877

EP - 882

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -