The impact of respiratory tract mimicking media on antifungal susceptibility testing.

Roya  Vahedi-Shahandashti; Isabella Hofer; Hollie Leighton; Joanne L Fothergill; Daniel R Neill; C Lass-Flörl

doi:10.1128/aac.00225-25

The impact of respiratory tract mimicking media on antifungal susceptibility testing.

Roya Vahedi-Shahandashti (Lead / Corresponding author), Isabella Hofer, Hollie Leighton, Joanne L Fothergill, Daniel R Neill, C Lass-Flörl

Molecular Microbiology

Research output: Contribution to journal › Article › peer-review

Abstract

The rise in antifungal resistance and changing epidemiology underscores the need to improve antifungal susceptibility testing. This study tested the standard medium RPMI₁₆₄₀ and healthy lung and sinus media against Aspergillus, Mucor, and Candida species. Candida showed greater variability in minimum inhibitory concentrations (MICs) compared with molds, with higher MICs in respiratory tract-mimicking media than in RPMI₁₆₄₀. Aspergillus terreus was the most affected mold, showing higher MICs for certain antifungals in these media.

Original language	English
Article number	e00225-25
Number of pages	7
Journal	Antimicrobial Agents and Chemotherapy
Volume	69
Issue number	8
Early online date	17 Jun 2025
DOIs	https://doi.org/10.1128/aac.00225-25
Publication status	Published - 6 Aug 2025

Keywords

antifungal resistance
antifungal susceptibility testing
broth microdilution
invasive fungal infections
respiratory tract disease

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/aac.00225-25Licence: CC BY

Final published version
Copyright © 2025 Vahedi-Shahandashti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Final published version, 258 KBLicence: CC BY

Cite this

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AU - Lass-Flörl, C

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KW - broth microdilution

KW - invasive fungal infections

KW - respiratory tract disease

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ER -

The impact of respiratory tract mimicking media on antifungal susceptibility testing.

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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