The impact of respiratory tract mimicking media on antifungal susceptibility testing.

Roya  Vahedi-Shahandashti; Isabella Hofer; Hollie Leighton; Joanne L Fothergill; Daniel R Neill; C Lass-Flörl

doi:10.1128/aac.00225-25

The impact of respiratory tract mimicking media on antifungal susceptibility testing.

Roya Vahedi-Shahandashti (Lead / Corresponding author)
, Isabella Hofer
, Hollie Leighton
, Joanne L Fothergill
, Daniel R Neill
, C Lass-Flörl

Molecular Microbiology

Research output: Contribution to journal › Article › peer-review

14 Downloads (Pure)

Abstract

The rise in antifungal resistance and changing epidemiology underscores the need to improve antifungal susceptibility testing. This study tested the standard medium RPMI₁₆₄₀ and healthy lung and sinus media against Aspergillus, Mucor, and Candida species. Candida showed greater variability in minimum inhibitory concentrations (MICs) compared with molds, with higher MICs in respiratory tract-mimicking media than in RPMI₁₆₄₀. Aspergillus terreus was the most affected mold, showing higher MICs for certain antifungals in these media.

Original language	English
Article number	e00225-25
Number of pages	7
Journal	Antimicrobial Agents and Chemotherapy
Volume	69
Issue number	8
Early online date	17 Jun 2025
DOIs	https://doi.org/10.1128/aac.00225-25
Publication status	Published - 6 Aug 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

antifungal resistance
antifungal susceptibility testing
broth microdilution
invasive fungal infections
respiratory tract disease

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

Access to Document

10.1128/aac.00225-25Licence: CC BY

Final published version
Copyright © 2025 Vahedi-Shahandashti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Final published version, 258 KBLicence: CC BY

Cite this

@article{0756c88b4bee4f8dadb48f0ef271d5ca,

title = "The impact of respiratory tract mimicking media on antifungal susceptibility testing.",

abstract = "The rise in antifungal resistance and changing epidemiology underscores the need to improve antifungal susceptibility testing. This study tested the standard medium RPMI1640 and healthy lung and sinus media against Aspergillus, Mucor, and Candida species. Candida showed greater variability in minimum inhibitory concentrations (MICs) compared with molds, with higher MICs in respiratory tract-mimicking media than in RPMI1640. Aspergillus terreus was the most affected mold, showing higher MICs for certain antifungals in these media.",

keywords = "antifungal resistance, antifungal susceptibility testing, broth microdilution, invasive fungal infections, respiratory tract disease",

author = "Roya Vahedi-Shahandashti and Isabella Hofer and Hollie Leighton and Fothergill, \{Joanne L\} and Neill, \{Daniel R\} and C Lass-Fl{\"o}rl",

year = "2025",

month = aug,

day = "6",

doi = "10.1128/aac.00225-25",

language = "English",

volume = "69",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "8",

}

TY - JOUR

T1 - The impact of respiratory tract mimicking media on antifungal susceptibility testing.

AU - Vahedi-Shahandashti, Roya

AU - Hofer, Isabella

AU - Leighton, Hollie

AU - Fothergill, Joanne L

AU - Neill, Daniel R

AU - Lass-Flörl, C

PY - 2025/8/6

Y1 - 2025/8/6

N2 - The rise in antifungal resistance and changing epidemiology underscores the need to improve antifungal susceptibility testing. This study tested the standard medium RPMI1640 and healthy lung and sinus media against Aspergillus, Mucor, and Candida species. Candida showed greater variability in minimum inhibitory concentrations (MICs) compared with molds, with higher MICs in respiratory tract-mimicking media than in RPMI1640. Aspergillus terreus was the most affected mold, showing higher MICs for certain antifungals in these media.

AB - The rise in antifungal resistance and changing epidemiology underscores the need to improve antifungal susceptibility testing. This study tested the standard medium RPMI1640 and healthy lung and sinus media against Aspergillus, Mucor, and Candida species. Candida showed greater variability in minimum inhibitory concentrations (MICs) compared with molds, with higher MICs in respiratory tract-mimicking media than in RPMI1640. Aspergillus terreus was the most affected mold, showing higher MICs for certain antifungals in these media.

KW - antifungal resistance

KW - antifungal susceptibility testing

KW - broth microdilution

KW - invasive fungal infections

KW - respiratory tract disease

UR - https://europepmc.org/articles/PMC12327005

U2 - 10.1128/aac.00225-25

DO - 10.1128/aac.00225-25

M3 - Article

C2 - 40525409

SN - 0066-4804

VL - 69

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 8

M1 - e00225-25

ER -

The impact of respiratory tract mimicking media on antifungal susceptibility testing.

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this