The influence of infusions of 1-desamino-8-D-arginine vasopressin (DDAVP) in vivo on the anticoagulant effect of recombinant hirudin (CGP39393) in vitro

S H Ibbotson, P J Grant, R Kerry, V S Findlay, C R Prentice

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Hirudin is a specific, potent inhibitor of thrombin that may be a valuable antithrombotic agent. The aim of this study was to investigate the hypothesis that the haemostatic effects of DDAVP counteract the coagulation defect induced by hirudin. The effect of DDAVP was studied in vivo on the anticoagulant action of recombinant hirudin (CGP39393) in vitro. Blood samples were taken at intervals from 10 normal volunteers infused with DDAVP. Factor VIII:C rose from (mean) 0.68 IU/ml before DDAVP to 2.19 and 2.16 IU/ml after 30 and 60 min infusion, respectively. Samples taken during DDAVP infusion showed a dose related decrease in the hirudin (0.5 and 1.0 microM) induced prolongation of the APTT, that occurred at FVIII:C concentrations of up to twice normal. At higher concentrations of hirudin no effect on the APTT occurred. These results demonstrate that DDAVP infusion elevates factor VIII:C levels with an associated significant reduction in the anticoagulant effect of hirudin in vitro.
    Original languageEnglish
    Pages (from-to)64-66
    Number of pages3
    JournalThrombosis and Haemostasis
    Volume65
    Issue number1
    Publication statusPublished - 23 Jan 1991

    Fingerprint

    Hirudins
    Deamino Arginine Vasopressin
    Anticoagulants
    Factor VIII
    Fibrinolytic Agents
    Hemostatics
    Thrombin
    In Vitro Techniques
    Healthy Volunteers

    Keywords

    • Anticoagulants
    • Infusions, Intravenous
    • Recombinant Proteins
    • Humans
    • Deamino Arginine Vasopressin
    • Hirudins
    • Factor VIII
    • Partial Thromboplastin Time

    Cite this

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    title = "The influence of infusions of 1-desamino-8-D-arginine vasopressin (DDAVP) in vivo on the anticoagulant effect of recombinant hirudin (CGP39393) in vitro",
    abstract = "Hirudin is a specific, potent inhibitor of thrombin that may be a valuable antithrombotic agent. The aim of this study was to investigate the hypothesis that the haemostatic effects of DDAVP counteract the coagulation defect induced by hirudin. The effect of DDAVP was studied in vivo on the anticoagulant action of recombinant hirudin (CGP39393) in vitro. Blood samples were taken at intervals from 10 normal volunteers infused with DDAVP. Factor VIII:C rose from (mean) 0.68 IU/ml before DDAVP to 2.19 and 2.16 IU/ml after 30 and 60 min infusion, respectively. Samples taken during DDAVP infusion showed a dose related decrease in the hirudin (0.5 and 1.0 microM) induced prolongation of the APTT, that occurred at FVIII:C concentrations of up to twice normal. At higher concentrations of hirudin no effect on the APTT occurred. These results demonstrate that DDAVP infusion elevates factor VIII:C levels with an associated significant reduction in the anticoagulant effect of hirudin in vitro.",
    keywords = "Anticoagulants, Infusions, Intravenous, Recombinant Proteins, Humans, Deamino Arginine Vasopressin, Hirudins, Factor VIII, Partial Thromboplastin Time",
    author = "Ibbotson, {S H} and Grant, {P J} and R Kerry and Findlay, {V S} and Prentice, {C R}",
    year = "1991",
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    The influence of infusions of 1-desamino-8-D-arginine vasopressin (DDAVP) in vivo on the anticoagulant effect of recombinant hirudin (CGP39393) in vitro. / Ibbotson, S H; Grant, P J; Kerry, R; Findlay, V S; Prentice, C R.

    In: Thrombosis and Haemostasis, Vol. 65, No. 1, 23.01.1991, p. 64-66.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The influence of infusions of 1-desamino-8-D-arginine vasopressin (DDAVP) in vivo on the anticoagulant effect of recombinant hirudin (CGP39393) in vitro

    AU - Ibbotson, S H

    AU - Grant, P J

    AU - Kerry, R

    AU - Findlay, V S

    AU - Prentice, C R

    PY - 1991/1/23

    Y1 - 1991/1/23

    N2 - Hirudin is a specific, potent inhibitor of thrombin that may be a valuable antithrombotic agent. The aim of this study was to investigate the hypothesis that the haemostatic effects of DDAVP counteract the coagulation defect induced by hirudin. The effect of DDAVP was studied in vivo on the anticoagulant action of recombinant hirudin (CGP39393) in vitro. Blood samples were taken at intervals from 10 normal volunteers infused with DDAVP. Factor VIII:C rose from (mean) 0.68 IU/ml before DDAVP to 2.19 and 2.16 IU/ml after 30 and 60 min infusion, respectively. Samples taken during DDAVP infusion showed a dose related decrease in the hirudin (0.5 and 1.0 microM) induced prolongation of the APTT, that occurred at FVIII:C concentrations of up to twice normal. At higher concentrations of hirudin no effect on the APTT occurred. These results demonstrate that DDAVP infusion elevates factor VIII:C levels with an associated significant reduction in the anticoagulant effect of hirudin in vitro.

    AB - Hirudin is a specific, potent inhibitor of thrombin that may be a valuable antithrombotic agent. The aim of this study was to investigate the hypothesis that the haemostatic effects of DDAVP counteract the coagulation defect induced by hirudin. The effect of DDAVP was studied in vivo on the anticoagulant action of recombinant hirudin (CGP39393) in vitro. Blood samples were taken at intervals from 10 normal volunteers infused with DDAVP. Factor VIII:C rose from (mean) 0.68 IU/ml before DDAVP to 2.19 and 2.16 IU/ml after 30 and 60 min infusion, respectively. Samples taken during DDAVP infusion showed a dose related decrease in the hirudin (0.5 and 1.0 microM) induced prolongation of the APTT, that occurred at FVIII:C concentrations of up to twice normal. At higher concentrations of hirudin no effect on the APTT occurred. These results demonstrate that DDAVP infusion elevates factor VIII:C levels with an associated significant reduction in the anticoagulant effect of hirudin in vitro.

    KW - Anticoagulants

    KW - Infusions, Intravenous

    KW - Recombinant Proteins

    KW - Humans

    KW - Deamino Arginine Vasopressin

    KW - Hirudins

    KW - Factor VIII

    KW - Partial Thromboplastin Time

    M3 - Article

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    EP - 66

    JO - Thrombosis and Haemostasis

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    SN - 0340-6245

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