The interaction of four-way DNA junctions with resolving enzymes

David M. J. Lilley

doi:10.1042/BST0380399

The interaction of four-way DNA junctions with resolving enzymes

David M. J. Lilley

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Four-way DNA (Holliday) junctions are resolved into duplex species by the action of the junction-resolving enzymes, nucleases selective for the structure of helical branchpoints. These have been isolated from bacteria and their phages, archaea, yeasts and mammals, including humans. They are all dimeric proteins that bind with high selectivity to DNA junctions and generate bilateral cleavage within the lifetime of the DNA-protein complex. Recent success in obtaining X-ray crystal structures of resolving enzymes bound to DNA junctions has revealed how the structural selectivity of these enzymes is achieved.

Original language	English
Pages (from-to)	399-403
Number of pages	5
Journal	Biochemical Society Transactions
Volume	38
DOIs	https://doi.org/10.1042/BST0380399
Publication status	Published - Apr 2010

Keywords

DNA repair
genetic recombination
Holiday junction
molecular recognition
nuclease
T4 ENDONUCLEASE VII
HOLLIDAY-JUNCTION
GENETIC-RECOMBINATION
T7 ENDONUCLEASE
CRYSTAL-STRUCTURE
MAMMALIAN-CELLS
GENE-3 ENDONUCLEASE
YEAST
RESOLUTION
REPAIR

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title = "The interaction of four-way DNA junctions with resolving enzymes",

abstract = "Four-way DNA (Holliday) junctions are resolved into duplex species by the action of the junction-resolving enzymes, nucleases selective for the structure of helical branchpoints. These have been isolated from bacteria and their phages, archaea, yeasts and mammals, including humans. They are all dimeric proteins that bind with high selectivity to DNA junctions and generate bilateral cleavage within the lifetime of the DNA-protein complex. Recent success in obtaining X-ray crystal structures of resolving enzymes bound to DNA junctions has revealed how the structural selectivity of these enzymes is achieved.",

keywords = "DNA repair, genetic recombination, Holiday junction, molecular recognition, nuclease, T4 ENDONUCLEASE VII, HOLLIDAY-JUNCTION, GENETIC-RECOMBINATION, T7 ENDONUCLEASE, CRYSTAL-STRUCTURE, MAMMALIAN-CELLS, GENE-3 ENDONUCLEASE, YEAST, RESOLUTION, REPAIR",

author = "Lilley, {David M. J.}",

year = "2010",

month = apr,

doi = "10.1042/BST0380399",

language = "English",

volume = "38",

pages = "399--403",

journal = "Biochemical Society Transactions",

issn = "0300-5127",

publisher = "Portland Press",

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TY - JOUR

T1 - The interaction of four-way DNA junctions with resolving enzymes

AU - Lilley, David M. J.

PY - 2010/4

Y1 - 2010/4

N2 - Four-way DNA (Holliday) junctions are resolved into duplex species by the action of the junction-resolving enzymes, nucleases selective for the structure of helical branchpoints. These have been isolated from bacteria and their phages, archaea, yeasts and mammals, including humans. They are all dimeric proteins that bind with high selectivity to DNA junctions and generate bilateral cleavage within the lifetime of the DNA-protein complex. Recent success in obtaining X-ray crystal structures of resolving enzymes bound to DNA junctions has revealed how the structural selectivity of these enzymes is achieved.

AB - Four-way DNA (Holliday) junctions are resolved into duplex species by the action of the junction-resolving enzymes, nucleases selective for the structure of helical branchpoints. These have been isolated from bacteria and their phages, archaea, yeasts and mammals, including humans. They are all dimeric proteins that bind with high selectivity to DNA junctions and generate bilateral cleavage within the lifetime of the DNA-protein complex. Recent success in obtaining X-ray crystal structures of resolving enzymes bound to DNA junctions has revealed how the structural selectivity of these enzymes is achieved.

KW - DNA repair

KW - genetic recombination

KW - Holiday junction

KW - molecular recognition

KW - nuclease

KW - T4 ENDONUCLEASE VII

KW - HOLLIDAY-JUNCTION

KW - GENETIC-RECOMBINATION

KW - T7 ENDONUCLEASE

KW - CRYSTAL-STRUCTURE

KW - MAMMALIAN-CELLS

KW - GENE-3 ENDONUCLEASE

KW - YEAST

KW - RESOLUTION

KW - REPAIR

U2 - 10.1042/BST0380399

DO - 10.1042/BST0380399

M3 - Article

C2 - 20298191

SN - 0300-5127

VL - 38

SP - 399

EP - 403

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

ER -

The interaction of four-way DNA junctions with resolving enzymes

Abstract

Keywords

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