The intraspinal release of prostaglandin E2 in a model of acute arthritis is accompanied by an up-regulation of cyclo-oxygenase-2 in the spinal cord

A. Ebersberger (Lead / Corresponding author), B. D. Grubb, H. L. Willingale, N. J. Gardiner, J. Nebe, H. G. Schaible

    Research output: Contribution to journalArticle

    108 Citations (Scopus)

    Abstract

    In anaesthetized rats, the intraspinal release of immunoreactive prostaglandin E2 was measured using antibody microprobes. We addressed the question of whether the release of immunoreactive prostaglandin E2 is altered during development of acute inflammation in the knee evoked by intra-articular injections of kaolin and carrageenan. We also examined cyclo-oxygenase-1 and cyclo-oxygenase-2 protein levels in the spinal cord during the development of inflammation using the same model of arthritis. Densitometric analysis of microprobes showed that basal release of immunoreactive prostaglandin E2 in the period 175-310min after kaolin was slightly higher than in the absence of inflammation. A pronounced enhancement of basal release of immunoreactive prostaglandin E2 was observed 430-530min after kaolin. Enhanced levels of immunoreactive prostaglandin E2 were observed throughout the dorsal and ventral horns. Release of immunoreactive prostaglandin E2 was not altered further by the application of innocuous and noxious pressure onto the inflamed knee. Western blot analysis revealed that cyclo-oxygenase-2 but not cyclo-oxygenase-1 protein levels were elevated in the spinal cords of animals with inflammation compared to normal animals. This effect was evident as early as 3h after the induction of arthritis. The maximum elevation of cyclo-oxygenase-2 protein levels (six-fold) was observed 12h after the induction of arthritis.The results show that there is a tonic release of immunoreactive prostaglandin E2 from the spinal cord following the induction of arthritis, which is accompanied by enhanced expression of cyclo-oxygenase-2 protein in the spinal cord. We suggest that intraspinal prostaglandins may play a role in inflammation-evoked central sensitizatlon of spinal cord neurons. Copyright (C) 1999 IBRO.

    Original languageEnglish
    Pages (from-to)775-781
    Number of pages7
    JournalNeuroscience
    Volume93
    Issue number2
    Early online date23 Jul 1999
    DOIs
    Publication statusPublished - Jul 1999

    Fingerprint

    Prostaglandin-Endoperoxide Synthases
    Dinoprostone
    Arthritis
    Spinal Cord
    Up-Regulation
    Kaolin
    Inflammation
    Knee
    Proteins
    Intra-Articular Injections
    Carrageenan
    Prostaglandins
    Western Blotting
    Neurons
    Pressure
    Antibodies

    Keywords

    • Cyclo-oxygenase
    • Inflammation
    • Intraspinal release
    • Pain
    • PGE

    Cite this

    Ebersberger, A. ; Grubb, B. D. ; Willingale, H. L. ; Gardiner, N. J. ; Nebe, J. ; Schaible, H. G. / The intraspinal release of prostaglandin E2 in a model of acute arthritis is accompanied by an up-regulation of cyclo-oxygenase-2 in the spinal cord. In: Neuroscience. 1999 ; Vol. 93, No. 2. pp. 775-781.
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    title = "The intraspinal release of prostaglandin E2 in a model of acute arthritis is accompanied by an up-regulation of cyclo-oxygenase-2 in the spinal cord",
    abstract = "In anaesthetized rats, the intraspinal release of immunoreactive prostaglandin E2 was measured using antibody microprobes. We addressed the question of whether the release of immunoreactive prostaglandin E2 is altered during development of acute inflammation in the knee evoked by intra-articular injections of kaolin and carrageenan. We also examined cyclo-oxygenase-1 and cyclo-oxygenase-2 protein levels in the spinal cord during the development of inflammation using the same model of arthritis. Densitometric analysis of microprobes showed that basal release of immunoreactive prostaglandin E2 in the period 175-310min after kaolin was slightly higher than in the absence of inflammation. A pronounced enhancement of basal release of immunoreactive prostaglandin E2 was observed 430-530min after kaolin. Enhanced levels of immunoreactive prostaglandin E2 were observed throughout the dorsal and ventral horns. Release of immunoreactive prostaglandin E2 was not altered further by the application of innocuous and noxious pressure onto the inflamed knee. Western blot analysis revealed that cyclo-oxygenase-2 but not cyclo-oxygenase-1 protein levels were elevated in the spinal cords of animals with inflammation compared to normal animals. This effect was evident as early as 3h after the induction of arthritis. The maximum elevation of cyclo-oxygenase-2 protein levels (six-fold) was observed 12h after the induction of arthritis.The results show that there is a tonic release of immunoreactive prostaglandin E2 from the spinal cord following the induction of arthritis, which is accompanied by enhanced expression of cyclo-oxygenase-2 protein in the spinal cord. We suggest that intraspinal prostaglandins may play a role in inflammation-evoked central sensitizatlon of spinal cord neurons. Copyright (C) 1999 IBRO.",
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    note = "The work was supported by the Deutsche Forschungsgemeinschaft (SFB 353) and a British Council Research Collaboration Grant awarded to Dr B. D. Grubb.",
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    The intraspinal release of prostaglandin E2 in a model of acute arthritis is accompanied by an up-regulation of cyclo-oxygenase-2 in the spinal cord. / Ebersberger, A. (Lead / Corresponding author); Grubb, B. D.; Willingale, H. L.; Gardiner, N. J.; Nebe, J.; Schaible, H. G.

    In: Neuroscience, Vol. 93, No. 2, 07.1999, p. 775-781.

    Research output: Contribution to journalArticle

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    T1 - The intraspinal release of prostaglandin E2 in a model of acute arthritis is accompanied by an up-regulation of cyclo-oxygenase-2 in the spinal cord

    AU - Ebersberger, A.

    AU - Grubb, B. D.

    AU - Willingale, H. L.

    AU - Gardiner, N. J.

    AU - Nebe, J.

    AU - Schaible, H. G.

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    N2 - In anaesthetized rats, the intraspinal release of immunoreactive prostaglandin E2 was measured using antibody microprobes. We addressed the question of whether the release of immunoreactive prostaglandin E2 is altered during development of acute inflammation in the knee evoked by intra-articular injections of kaolin and carrageenan. We also examined cyclo-oxygenase-1 and cyclo-oxygenase-2 protein levels in the spinal cord during the development of inflammation using the same model of arthritis. Densitometric analysis of microprobes showed that basal release of immunoreactive prostaglandin E2 in the period 175-310min after kaolin was slightly higher than in the absence of inflammation. A pronounced enhancement of basal release of immunoreactive prostaglandin E2 was observed 430-530min after kaolin. Enhanced levels of immunoreactive prostaglandin E2 were observed throughout the dorsal and ventral horns. Release of immunoreactive prostaglandin E2 was not altered further by the application of innocuous and noxious pressure onto the inflamed knee. Western blot analysis revealed that cyclo-oxygenase-2 but not cyclo-oxygenase-1 protein levels were elevated in the spinal cords of animals with inflammation compared to normal animals. This effect was evident as early as 3h after the induction of arthritis. The maximum elevation of cyclo-oxygenase-2 protein levels (six-fold) was observed 12h after the induction of arthritis.The results show that there is a tonic release of immunoreactive prostaglandin E2 from the spinal cord following the induction of arthritis, which is accompanied by enhanced expression of cyclo-oxygenase-2 protein in the spinal cord. We suggest that intraspinal prostaglandins may play a role in inflammation-evoked central sensitizatlon of spinal cord neurons. Copyright (C) 1999 IBRO.

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