The isolation, purifcation and biological activity of a novel antibacterial compound produced by Pseudomonas stutzeri

Bushra Uzair, Nuzhat Ahmed, Viqar Uddin Ahmad, Faryal Vali Mohammad, David H. Edwards

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    A novel compound designated zafrin [4b-methyl-5, 6, 7, 8 tetrahydro-1 (4b-H)-phenanthrenone] was isolated from a crude extract of a marine bacterium identified as Pseudomonas stutzeri. Zafrin showed strong antibacterial activity against both Gram-positive and Gram-negative bacteria. The compound was purified and its structure was elucidated by spectroscopic methods including 1Hnuclear magnetic resonance (NMR), 13C-NMR, 1D-NMR and 2D-NMR spectroscopy. It could be demonstrated that a purified solution of zafrin was active against several human pathogens, including Staphylococcus aureus, and Salmonella typhi. By contrast, zafrin did not inhibit the growth of eukaryotic organisms Candida albicans and Schizosaccharomyces pombe. The minimal inhibitory concentration for Gram-positive bacteria ranged from 50 to 75 mgmL 1 and varied between 75 and 125 mgmL 1 for Gram-negative bacteria. Zafrin lysed Bacillus subtilis cells grown in an osmotically protected medium, suggesting that it does not act upon the cell wall. Further investigation using B. subtilis indicated that the compound is bactericidal and is likely to target the cell membrane.
    Original languageEnglish
    Pages (from-to)243-250
    Number of pages8
    JournalFEMS Microbiology Letters
    Volume279
    Issue number2
    DOIs
    Publication statusPublished - Feb 2008

    Keywords

    • Antibiotic
    • Pseudomonas sp.
    • Antibacterial activity
    • Marine bacteria
    • Zafrin

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