The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells

Sebastian Schuck, Ana Soloaga, Gerhard Schratt, J. Simon C. Arthur, Alfred Nordheim (Lead / Corresponding author)

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    46 Citations (Scopus)

    Abstract

    Background: The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos

    Results: Analysing the signalling events following stimulation of mouse embryonic stem cells with serum and lysophosphatidic acid, we show that the extracellular signal-regulated kinase (ERK) pathway is involved in mediating c-fos induction. We demonstrate that the ERK-activated kinase MSK1 is required for full c-fos promoter activation, as well as for the phosphorylation of cAMPresponsive element (CRE) binding proteins. We propose that MSK1 contributes to ERK-mediated c-fos promoter activation by targeting CRE binding proteins. 

    Conclusion: These results show that MSK1 is an important ERK-activated mediator of mitogenstimulated c-fos induction. In addition, they indicate that MSK1 could act through CRE binding proteins to achieve c-fos promoter activation. Thus, they further our understanding of the complex regulation of the model immediate-early gene c-fos.

    Original languageEnglish
    Article number6
    Number of pages8
    JournalBMC Molecular Biology
    Volume4
    DOIs
    Publication statusPublished - 26 May 2003

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