The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells

Sebastian Schuck; Ana Soloaga; Gerhard Schratt; J. Simon C. Arthur; Alfred Nordheim

doi:10.1186/1471-2199-4-6

The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells

Sebastian Schuck, Ana Soloaga, Gerhard Schratt, J. Simon C. Arthur, Alfred Nordheim (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Background: The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos.

Results: Analysing the signalling events following stimulation of mouse embryonic stem cells with serum and lysophosphatidic acid, we show that the extracellular signal-regulated kinase (ERK) pathway is involved in mediating c-fos induction. We demonstrate that the ERK-activated kinase MSK1 is required for full c-fos promoter activation, as well as for the phosphorylation of cAMPresponsive element (CRE) binding proteins. We propose that MSK1 contributes to ERK-mediated c-fos promoter activation by targeting CRE binding proteins.

Conclusion: These results show that MSK1 is an important ERK-activated mediator of mitogenstimulated c-fos induction. In addition, they indicate that MSK1 could act through CRE binding proteins to achieve c-fos promoter activation. Thus, they further our understanding of the complex regulation of the model immediate-early gene c-fos.

Original language	English
Article number	6
Number of pages	8
Journal	BMC Molecular Biology
Volume	4
DOIs	https://doi.org/10.1186/1471-2199-4-6
Publication status	Published - 26 May 2003

ASJC Scopus subject areas

Molecular Biology

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title = "The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells",

abstract = "Background: The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos. Results: Analysing the signalling events following stimulation of mouse embryonic stem cells with serum and lysophosphatidic acid, we show that the extracellular signal-regulated kinase (ERK) pathway is involved in mediating c-fos induction. We demonstrate that the ERK-activated kinase MSK1 is required for full c-fos promoter activation, as well as for the phosphorylation of cAMPresponsive element (CRE) binding proteins. We propose that MSK1 contributes to ERK-mediated c-fos promoter activation by targeting CRE binding proteins. Conclusion: These results show that MSK1 is an important ERK-activated mediator of mitogenstimulated c-fos induction. In addition, they indicate that MSK1 could act through CRE binding proteins to achieve c-fos promoter activation. Thus, they further our understanding of the complex regulation of the model immediate-early gene c-fos.",

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T1 - The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells

AU - Schuck, Sebastian

AU - Soloaga, Ana

AU - Schratt, Gerhard

AU - Arthur, J. Simon C.

AU - Nordheim, Alfred

PY - 2003/5/26

Y1 - 2003/5/26

N2 - Background: The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos. Results: Analysing the signalling events following stimulation of mouse embryonic stem cells with serum and lysophosphatidic acid, we show that the extracellular signal-regulated kinase (ERK) pathway is involved in mediating c-fos induction. We demonstrate that the ERK-activated kinase MSK1 is required for full c-fos promoter activation, as well as for the phosphorylation of cAMPresponsive element (CRE) binding proteins. We propose that MSK1 contributes to ERK-mediated c-fos promoter activation by targeting CRE binding proteins. Conclusion: These results show that MSK1 is an important ERK-activated mediator of mitogenstimulated c-fos induction. In addition, they indicate that MSK1 could act through CRE binding proteins to achieve c-fos promoter activation. Thus, they further our understanding of the complex regulation of the model immediate-early gene c-fos.

AB - Background: The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos. Results: Analysing the signalling events following stimulation of mouse embryonic stem cells with serum and lysophosphatidic acid, we show that the extracellular signal-regulated kinase (ERK) pathway is involved in mediating c-fos induction. We demonstrate that the ERK-activated kinase MSK1 is required for full c-fos promoter activation, as well as for the phosphorylation of cAMPresponsive element (CRE) binding proteins. We propose that MSK1 contributes to ERK-mediated c-fos promoter activation by targeting CRE binding proteins. Conclusion: These results show that MSK1 is an important ERK-activated mediator of mitogenstimulated c-fos induction. In addition, they indicate that MSK1 could act through CRE binding proteins to achieve c-fos promoter activation. Thus, they further our understanding of the complex regulation of the model immediate-early gene c-fos.

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The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells

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