The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Nicolas Loyer; Jens Januschke

doi:10.1038/s41467-018-06276-0

The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Nicolas Loyer, Jens Januschke (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

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Abstract

Controlling the orientation of cell division is important in the context of cell fate choices and tissue morphogenesis. However, the mechanisms providing the required positional information remain incompletely understood. Here we use stem cells of the Drosophila larval brain that stably maintain their axis of polarity and division between cell cycles to identify cues that orient cell division. Using live cell imaging of cultured brains, laser ablation and genetics, we reveal that division axis maintenance relies on their last-born daughter cell. We propose that, in addition to known intrinsic cues, stem cells in the developing fly brain are polarized by an extrinsic signal. We further find that division axis maintenance allows neuroblasts to maximize their contact area with glial cells known to provide protective and proliferative signals to neuroblasts.

Original language	English
Article number	3745
Pages (from-to)	1-12
Number of pages	12
Journal	Nature Communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-06276-0
Publication status	Published - 14 Sept 2018

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/s41467-018-06276-0Licence: CC BY

Final Published Version
© The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 3.49 MBLicence: CC BY

Cite this

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title = "The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts",

abstract = "Controlling the orientation of cell division is important in the context of cell fate choices and tissue morphogenesis. However, the mechanisms providing the required positional information remain incompletely understood. Here we use stem cells of the Drosophila larval brain that stably maintain their axis of polarity and division between cell cycles to identify cues that orient cell division. Using live cell imaging of cultured brains, laser ablation and genetics, we reveal that division axis maintenance relies on their last-born daughter cell. We propose that, in addition to known intrinsic cues, stem cells in the developing fly brain are polarized by an extrinsic signal. We further find that division axis maintenance allows neuroblasts to maximize their contact area with glial cells known to provide protective and proliferative signals to neuroblasts.",

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note = " Work in J.J.{\textquoteright}s laboratory is supported by a Sir Henry Dale fellowship from Wellcome and the Royal Society (100031Z/12/Z and 100031Z/12/A). The tissue imaging facility is supported by the grant WT101468 from Wellcome.",

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AU - Januschke, Jens

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AB - Controlling the orientation of cell division is important in the context of cell fate choices and tissue morphogenesis. However, the mechanisms providing the required positional information remain incompletely understood. Here we use stem cells of the Drosophila larval brain that stably maintain their axis of polarity and division between cell cycles to identify cues that orient cell division. Using live cell imaging of cultured brains, laser ablation and genetics, we reveal that division axis maintenance relies on their last-born daughter cell. We propose that, in addition to known intrinsic cues, stem cells in the developing fly brain are polarized by an extrinsic signal. We further find that division axis maintenance allows neuroblasts to maximize their contact area with glial cells known to provide protective and proliferative signals to neuroblasts.

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The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Abstract

ASJC Scopus subject areas

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Recycling Polarity - Mechanisms Controlling Stem Cell Polarity in Consecutive Divisions in the Developing Drosophila Central Nervous System (Sir Henry Dale Fellowship)

Principles of asymmetric cell division

Asymmetric division of Drosophila neural stem cells

Januschke, Jens

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The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Abstract

ASJC Scopus subject areas

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Projects

Recycling Polarity - Mechanisms Controlling Stem Cell Polarity in Consecutive Divisions in the Developing Drosophila Central Nervous System (Sir Henry Dale Fellowship)

Activities

Principles of asymmetric cell division

Asymmetric division of Drosophila neural stem cells

Profiles

Januschke, Jens

Cite this