The leukocytic reaction of the human uterine cervix

L. A. Thompson, C. L. R. Barratt, A. E. Bolton, I. D. Cooke

Research output: Contribution to journalArticle

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Abstract

Problem: Sperm interaction with the immune system of the human cervix is poorly understood.

Method: The leukocytic response of the human cervix to sperm was examined in a closely monitored patient population (N = 10), using monoclonal antibody cell identification techniques. Baseline data were collected from both cervical mucus and smears sampled before treatment by donor insemination. Donor insemination was timed to coincide with ovulation by monitoring plasma LH concentrations twice daily. Following insemination the numbers of leukocytes were recorded in cervical mucus and smear samples taken over a 24-h period relative to the time of treatment. Controls treated with "pure sperm," seminal plasma, cryopreservative, and cervical smearing alone were also included in the study.

Results: Only those women treated with sperm cells exhibited substantial elevations in leukocyte numbers following inseminations. Additionally, serial cervical smearing induced an inflammatory response of the cervix. In all the women, the neutrophil was the predominant leukocyte of the cervix both during the baseline and treatment periods (median values ranged from 77 to 86%). Macrophages, T-helper lymphocytes, and T-suppressor lymphocytes were also detected, but only in low numbers (2-10.6%). Two patients and one control ("pure sperm") became pregnant during their study cycle.

Conclusions: We conclude that the leukocytic reaction is a physiological response of the cervix to sperm, the function of which remains to be established.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalAmerican Journal of Reproductive Immunology
Volume28
Issue number2
Publication statusPublished - Sep 1992

Fingerprint

Cervix Uteri
Spermatozoa
Insemination
Cervix Mucus
Vaginal Smears
Helper-Inducer T-Lymphocytes
Leukocyte Count
Tissue Donors
Ovulation
Semen
Immune System
Neutrophils
Leukocytes
Therapeutics
Macrophages
Monoclonal Antibodies
Lymphocytes
Population

Keywords

  • Cell Movement
  • Cervix Mucus/cytology
  • Cervix Uteri/immunology
  • Female
  • Humans
  • Insemination, Artificial, Heterologous
  • Leukocyte Count
  • Leukocytes/immunology
  • Luteinizing Hormone/blood
  • Male
  • Spermatozoa/immunology
  • Time Factors

Cite this

Thompson, L. A. ; Barratt, C. L. R. ; Bolton, A. E. ; Cooke, I. D. / The leukocytic reaction of the human uterine cervix. In: American Journal of Reproductive Immunology. 1992 ; Vol. 28, No. 2. pp. 85-89.
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abstract = "Problem: Sperm interaction with the immune system of the human cervix is poorly understood.Method: The leukocytic response of the human cervix to sperm was examined in a closely monitored patient population (N = 10), using monoclonal antibody cell identification techniques. Baseline data were collected from both cervical mucus and smears sampled before treatment by donor insemination. Donor insemination was timed to coincide with ovulation by monitoring plasma LH concentrations twice daily. Following insemination the numbers of leukocytes were recorded in cervical mucus and smear samples taken over a 24-h period relative to the time of treatment. Controls treated with {"}pure sperm,{"} seminal plasma, cryopreservative, and cervical smearing alone were also included in the study.Results: Only those women treated with sperm cells exhibited substantial elevations in leukocyte numbers following inseminations. Additionally, serial cervical smearing induced an inflammatory response of the cervix. In all the women, the neutrophil was the predominant leukocyte of the cervix both during the baseline and treatment periods (median values ranged from 77 to 86{\%}). Macrophages, T-helper lymphocytes, and T-suppressor lymphocytes were also detected, but only in low numbers (2-10.6{\%}). Two patients and one control ({"}pure sperm{"}) became pregnant during their study cycle.Conclusions: We conclude that the leukocytic reaction is a physiological response of the cervix to sperm, the function of which remains to be established.",
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Thompson, LA, Barratt, CLR, Bolton, AE & Cooke, ID 1992, 'The leukocytic reaction of the human uterine cervix', American Journal of Reproductive Immunology, vol. 28, no. 2, pp. 85-89.

The leukocytic reaction of the human uterine cervix. / Thompson, L. A.; Barratt, C. L. R.; Bolton, A. E.; Cooke, I. D.

In: American Journal of Reproductive Immunology, Vol. 28, No. 2, 09.1992, p. 85-89.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The leukocytic reaction of the human uterine cervix

AU - Thompson, L. A.

AU - Barratt, C. L. R.

AU - Bolton, A. E.

AU - Cooke, I. D.

PY - 1992/9

Y1 - 1992/9

N2 - Problem: Sperm interaction with the immune system of the human cervix is poorly understood.Method: The leukocytic response of the human cervix to sperm was examined in a closely monitored patient population (N = 10), using monoclonal antibody cell identification techniques. Baseline data were collected from both cervical mucus and smears sampled before treatment by donor insemination. Donor insemination was timed to coincide with ovulation by monitoring plasma LH concentrations twice daily. Following insemination the numbers of leukocytes were recorded in cervical mucus and smear samples taken over a 24-h period relative to the time of treatment. Controls treated with "pure sperm," seminal plasma, cryopreservative, and cervical smearing alone were also included in the study.Results: Only those women treated with sperm cells exhibited substantial elevations in leukocyte numbers following inseminations. Additionally, serial cervical smearing induced an inflammatory response of the cervix. In all the women, the neutrophil was the predominant leukocyte of the cervix both during the baseline and treatment periods (median values ranged from 77 to 86%). Macrophages, T-helper lymphocytes, and T-suppressor lymphocytes were also detected, but only in low numbers (2-10.6%). Two patients and one control ("pure sperm") became pregnant during their study cycle.Conclusions: We conclude that the leukocytic reaction is a physiological response of the cervix to sperm, the function of which remains to be established.

AB - Problem: Sperm interaction with the immune system of the human cervix is poorly understood.Method: The leukocytic response of the human cervix to sperm was examined in a closely monitored patient population (N = 10), using monoclonal antibody cell identification techniques. Baseline data were collected from both cervical mucus and smears sampled before treatment by donor insemination. Donor insemination was timed to coincide with ovulation by monitoring plasma LH concentrations twice daily. Following insemination the numbers of leukocytes were recorded in cervical mucus and smear samples taken over a 24-h period relative to the time of treatment. Controls treated with "pure sperm," seminal plasma, cryopreservative, and cervical smearing alone were also included in the study.Results: Only those women treated with sperm cells exhibited substantial elevations in leukocyte numbers following inseminations. Additionally, serial cervical smearing induced an inflammatory response of the cervix. In all the women, the neutrophil was the predominant leukocyte of the cervix both during the baseline and treatment periods (median values ranged from 77 to 86%). Macrophages, T-helper lymphocytes, and T-suppressor lymphocytes were also detected, but only in low numbers (2-10.6%). Two patients and one control ("pure sperm") became pregnant during their study cycle.Conclusions: We conclude that the leukocytic reaction is a physiological response of the cervix to sperm, the function of which remains to be established.

KW - Cell Movement

KW - Cervix Mucus/cytology

KW - Cervix Uteri/immunology

KW - Female

KW - Humans

KW - Insemination, Artificial, Heterologous

KW - Leukocyte Count

KW - Leukocytes/immunology

KW - Luteinizing Hormone/blood

KW - Male

KW - Spermatozoa/immunology

KW - Time Factors

M3 - Article

VL - 28

SP - 85

EP - 89

JO - American Journal of Reproductive Immunology

JF - American Journal of Reproductive Immunology

SN - 1046-7408

IS - 2

ER -