The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression

Onur Sen, Adrian T. Saurin, Jonathan M.G. Higgins (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    1 Citation (Scopus)
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    Abstract

    SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 μM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 μM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.

    Original languageEnglish
    Article number7898
    Pages (from-to)1-8
    Number of pages8
    JournalScientific Reports
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 21 May 2018

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    DNA Damage
    Cell Cycle
    Coloring Agents
    Time-Lapse Imaging
    Cyclin B1
    Cell Nucleus Active Transport
    Poisons
    DNA
    DNA Probes
    Fluorescent Dyes
    Cultured Cells

    Cite this

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    abstract = "SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 μM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 μM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.",
    author = "Onur Sen and Saurin, {Adrian T.} and Higgins, {Jonathan M.G.}",
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    The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression. / Sen, Onur ; Saurin, Adrian T.; Higgins, Jonathan M.G. (Lead / Corresponding author).

    In: Scientific Reports, Vol. 8, No. 1, 7898, 21.05.2018, p. 1-8.

    Research output: Contribution to journalArticle

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    AU - Sen, Onur

    AU - Saurin, Adrian T.

    AU - Higgins, Jonathan M.G.

    N1 - We are very grateful to Drs Alex Laude, Glyn Nelson and Rolando Berlinguer Palmini of the Newcastle University Bio-Imaging Facility for their help. This study was funded by a Wellcome Trust Investigator Award and a Royal Society Wolfson Research Merit Award to J.M.G.H. and an award from Cancer Research UK (C47320/A21229) to A.T.S.

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