The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression

Onur Sen, Adrian T. Saurin, Jonathan M.G. Higgins (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    2 Citations (Scopus)
    132 Downloads (Pure)

    Abstract

    SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 μM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 μM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.

    Original languageEnglish
    Article number7898
    Pages (from-to)1-8
    Number of pages8
    JournalScientific Reports
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 21 May 2018

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