The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression

Onur  Sen; Adrian T. Saurin; Jonathan M.G.  Higgins

doi:10.1038/s41598-018-26307-6

The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression

Onur Sen
, Adrian T. Saurin
, Jonathan M.G. Higgins (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

323 Downloads (Pure)

Abstract

SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 μM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 μM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.

Original language	English
Article number	7898
Pages (from-to)	1-8
Number of pages	8
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-26307-6
Publication status	Published - 21 May 2018

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2018
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title = "The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression",

abstract = "SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 μM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 μM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.",

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note = "We are very grateful to Drs Alex Laude, Glyn Nelson and Rolando Berlinguer Palmini of the Newcastle University Bio-Imaging Facility for their help. This study was funded by a Wellcome Trust Investigator Award and a Royal Society Wolfson Research Merit Award to J.M.G.H. and an award from Cancer Research UK (C47320/A21229) to A.T.S.",

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AB - SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 μM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 μM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.

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The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression

Abstract

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