The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

Judit Remenyi; Sarah Bajan; Frances V. Fuller-Pace; J. Simon C Arthur; Gyorgy Hutvagner

doi:10.1038/srep22848

The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

Judit Remenyi, Sarah Bajan, Frances V. Fuller-Pace (Lead / Corresponding author), J. Simon C Arthur (Lead / Corresponding author), Gyorgy Hutvagner (Lead / Corresponding author)

Cell Signalling and Immunology

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Abstract

miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.

Original language	English
Article number	22848
Pages (from-to)	1-13
Number of pages	13
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep22848
Publication status	Published - 7 Mar 2016

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title = "The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132",

abstract = "miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.",

author = "Judit Remenyi and Sarah Bajan and Fuller-Pace, {Frances V.} and Arthur, {J. Simon C} and Gyorgy Hutvagner",

note = "This work was supported by the Wellcome Trust CDF, EU FP6 SIROCCO project. G.H is an ARC Future Fellow. J.R. was funded by a grant from Breast Cancer Now (2010NOVPR16-previously known as Breast Cancer Campaign).",

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AU - Arthur, J. Simon C

AU - Hutvagner, Gyorgy

N1 - This work was supported by the Wellcome Trust CDF, EU FP6 SIROCCO project. G.H is an ARC Future Fellow. J.R. was funded by a grant from Breast Cancer Now (2010NOVPR16-previously known as Breast Cancer Campaign).

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N2 - miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.

AB - miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.

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The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

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