The major glutathione S-transferase in salmonid fish livers is homologous to the mammalian pi-class GST

R J Dominey, I A Nimmo, A D Cronshaw, J D Hayes

    Research output: Contribution to journalArticle

    62 Citations (Scopus)

    Abstract

    1. The hepatic glutathione S-transferase (GST) isoenzymes were isolated and characterized from salmon, sea trout and rainbow trout. 2. In all three species the predominant GST expressed comprised subunits of Mr 24,800. These subunits each co-migrated with the rat pi-class Yf polypeptide during SDS/polyacrylamide gel electrophoresis. 3. Western blotting experiments demonstrated immunochemical cross-reactivity between the major salmonid and the rat pi-class GSTs. 4. The salmon GST of subunit Mr 24,800 was digested with cyanogen bromide and the peptides, once purified by reverse-phase HPLC, were subjected to automated amino acid sequencing. 5. Over the region sequenced, the salmon GST possessed about 65% homology with the rat and human pi-class GST.

    Original languageEnglish
    Pages (from-to)93-8
    Number of pages6
    JournalComparative Biochemistry and Physiology Part B: Comparative Biochemistry
    Volume100
    Issue number1
    Publication statusPublished - 1991

    Keywords

    • Amino Acid Sequence
    • Animals
    • Glutathione Transferase/chemistry
    • Humans
    • Isoenzymes/chemistry
    • Liver/enzymology
    • Mammals
    • Molecular Sequence Data
    • Molecular Weight
    • Protein Conformation
    • Salmonidae/metabolism
    • Sequence Homology, Nucleic Acid
    • Species Specificity

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