TY - JOUR
T1 - The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver
AU - Cohen, Patricia T.W.
AU - Schelling, Donald L.
AU - da Cruz e Silva, Odete B.
AU - Barker, Hazel M.
AU - Cohen, Philip
PY - 1989/6/1
Y1 - 1989/6/1
N2 - The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1α from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.
AB - The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1α from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.
KW - (Rabbit liver)
KW - Amino acid sequence
KW - cDNA cloning
KW - Nucleic acid sequence
KW - Peptide mapping
KW - Protein phosphatase
UR - http://www.scopus.com/inward/record.url?scp=0024313038&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(89)90181-4
DO - 10.1016/0167-4781(89)90181-4
M3 - Article
C2 - 2541784
AN - SCOPUS:0024313038
VL - 1008
SP - 125
EP - 128
JO - Biochimica et Biophysica Acta-Gene Structure and Expression
JF - Biochimica et Biophysica Acta-Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -