The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver

Patricia T.W. Cohen; Donald L. Schelling; Odete B. da Cruz e Silva; Hazel M. Barker; Philip Cohen

doi:10.1016/0167-4781(89)90181-4

The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver

Patricia T.W. Cohen, Donald L. Schelling, Odete B. da Cruz e Silva, Hazel M. Barker, Philip Cohen

MRC PPU

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25 Citations (Scopus)

Abstract

The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1α from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.

Original language	English
Pages (from-to)	125-128
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1008
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(89)90181-4
Publication status	Published - 1 Jun 1989

Keywords

(Rabbit liver)
Amino acid sequence
cDNA cloning
Nucleic acid sequence
Peptide mapping
Protein phosphatase

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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abstract = "The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1α from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.",

keywords = "(Rabbit liver), Amino acid sequence, cDNA cloning, Nucleic acid sequence, Peptide mapping, Protein phosphatase",

author = "Cohen, {Patricia T.W.} and Schelling, {Donald L.} and {da Cruz e Silva}, {Odete B.} and Barker, {Hazel M.} and Philip Cohen",

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T1 - The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver

AU - Cohen, Patricia T.W.

AU - Schelling, Donald L.

AU - da Cruz e Silva, Odete B.

AU - Barker, Hazel M.

AU - Cohen, Philip

PY - 1989/6/1

Y1 - 1989/6/1

N2 - The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1α from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.

AB - The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1α from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.

KW - (Rabbit liver)

KW - Amino acid sequence

KW - cDNA cloning

KW - Nucleic acid sequence

KW - Peptide mapping

KW - Protein phosphatase

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The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver

Abstract

Keywords

ASJC Scopus subject areas

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