The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver

Patricia T.W. Cohen, Donald L. Schelling, Odete B. da Cruz e Silva, Hazel M. Barker, Philip Cohen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1α from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1008
Issue number1
DOIs
Publication statusPublished - 1 Jun 1989

Fingerprint

Protein Phosphatase 1
Liver
Muscle
Skeletal Muscle
Genes
Rabbits
Muscle Proteins
Catalytic Domain
Phosphoprotein Phosphatases
Substrate Specificity
Electrophoretic mobility
Amino Acid Sequence
Complementary DNA
Clone Cells
Peptides
Amino Acids
Substrates

Keywords

  • (Rabbit liver)
  • Amino acid sequence
  • cDNA cloning
  • Nucleic acid sequence
  • Peptide mapping
  • Protein phosphatase

Cite this

Cohen, Patricia T.W. ; Schelling, Donald L. ; da Cruz e Silva, Odete B. ; Barker, Hazel M. ; Cohen, Philip. / The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver. In: BBA - Gene Structure and Expression. 1989 ; Vol. 1008, No. 1. pp. 125-128.
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The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver. / Cohen, Patricia T.W.; Schelling, Donald L.; da Cruz e Silva, Odete B.; Barker, Hazel M.; Cohen, Philip.

In: BBA - Gene Structure and Expression, Vol. 1008, No. 1, 01.06.1989, p. 125-128.

Research output: Contribution to journalArticle

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AU - Cohen, Philip

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