The mammalian ULK1 complex and autophagy initiation

Maria Zachari, Ian G. Ganley (Lead / Corresponding author)

Research output: Contribution to journalReview article

45 Citations (Scopus)
98 Downloads (Pure)

Abstract

Autophagy is a vital lysosomal degradation pathway that serves as a quality control mechanism. It rids the cell of damaged, toxic or excess cellular components, which if left to persist could be detrimental to the cell. It also serves as a recycling pathway to maintain protein synthesis under starvation conditions. A key initial event in autophagy is formation of the autophagosome, the unique double-membrane organelle that engulfs the cytosolic cargo destined for degradation. This step is mediated by the serine/threonine protein kinase ULK1, which functions in a complex with at least three protein partners: FIP200, ATG13, and ATG101. In this chapter, we will focus on the regulation of the ULK1 complex during autophagy initiation. The complex pattern of upstream pathways that converge on ULK1 suggests that this complex acts as a node, converting multiple signals into autophagosome formation. Here we will review our current understanding of this regulation and in turn discuss what happens downstream, once the ULK1 complex becomes activated.
Original languageEnglish
Pages (from-to)585-596
Number of pages12
JournalEssays in Biochemistry
Volume61
Issue number6
DOIs
Publication statusPublished - 12 Dec 2017

Fingerprint

Autophagy
Degradation
Poisons
Protein-Serine-Threonine Kinases
Quality control
Recycling
Proteins
Starvation
Membranes
Quality Control
Organelles
Autophagosomes

Keywords

  • Autophagy
  • ULK1
  • ATG13
  • FIP200
  • ATG101
  • mTOR
  • Kinase
  • Autophagosome
  • Phagophore

Cite this

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title = "The mammalian ULK1 complex and autophagy initiation",
abstract = "Autophagy is a vital lysosomal degradation pathway that serves as a quality control mechanism. It rids the cell of damaged, toxic or excess cellular components, which if left to persist could be detrimental to the cell. It also serves as a recycling pathway to maintain protein synthesis under starvation conditions. A key initial event in autophagy is formation of the autophagosome, the unique double-membrane organelle that engulfs the cytosolic cargo destined for degradation. This step is mediated by the serine/threonine protein kinase ULK1, which functions in a complex with at least three protein partners: FIP200, ATG13, and ATG101. In this chapter, we will focus on the regulation of the ULK1 complex during autophagy initiation. The complex pattern of upstream pathways that converge on ULK1 suggests that this complex acts as a node, converting multiple signals into autophagosome formation. Here we will review our current understanding of this regulation and in turn discuss what happens downstream, once the ULK1 complex becomes activated.",
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The mammalian ULK1 complex and autophagy initiation. / Zachari, Maria; Ganley, Ian G. (Lead / Corresponding author).

In: Essays in Biochemistry, Vol. 61, No. 6, 12.12.2017, p. 585-596.

Research output: Contribution to journalReview article

TY - JOUR

T1 - The mammalian ULK1 complex and autophagy initiation

AU - Zachari, Maria

AU - Ganley, Ian G.

N1 - We thank the Ganley Lab for critical reading of the manuscript. This work was supported by the Medical Research Council, UK (MC_UU_12016/4).

PY - 2017/12/12

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AB - Autophagy is a vital lysosomal degradation pathway that serves as a quality control mechanism. It rids the cell of damaged, toxic or excess cellular components, which if left to persist could be detrimental to the cell. It also serves as a recycling pathway to maintain protein synthesis under starvation conditions. A key initial event in autophagy is formation of the autophagosome, the unique double-membrane organelle that engulfs the cytosolic cargo destined for degradation. This step is mediated by the serine/threonine protein kinase ULK1, which functions in a complex with at least three protein partners: FIP200, ATG13, and ATG101. In this chapter, we will focus on the regulation of the ULK1 complex during autophagy initiation. The complex pattern of upstream pathways that converge on ULK1 suggests that this complex acts as a node, converting multiple signals into autophagosome formation. Here we will review our current understanding of this regulation and in turn discuss what happens downstream, once the ULK1 complex becomes activated.

KW - Autophagy

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KW - Autophagosome

KW - Phagophore

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