The minimum M3-M4 loop length of neurotransmitteractivated pentameric receptors is critical for the structural integrity of cytoplasmic portals

Daniel T. Baptista-Hon, Tarek Z. Deeb, Jeremy J. Lambert, John A. Peters, Tim G. Hales (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Background: Residue 436 within 5-HT3A cytoplasmic portals determines single channel conductance (?) and rectification. Results: M3-M4 loop truncation to 75 residues spared inward rectification; truncation to 70 abolished rectification and increased -. Human Cys-loop neurotransmitter receptor M3-M4 loops all exceed 70 residues. Conclusion: M3-M4 lengths of >70 are critical. Significance: Cytoplasmic portals may be essential for normal function of Cys-loop neurotransmitter receptors.
    Original languageEnglish
    Pages (from-to)21558-21568
    Number of pages11
    JournalJournal of Biological Chemistry
    Volume288
    Issue number30
    DOIs
    Publication statusPublished - 26 Jul 2013

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    Cysteine Loop Ligand-Gated Ion Channel Receptors
    Neurotransmitter Receptor
    Structural integrity

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    abstract = "Background: Residue 436 within 5-HT3A cytoplasmic portals determines single channel conductance (?) and rectification. Results: M3-M4 loop truncation to 75 residues spared inward rectification; truncation to 70 abolished rectification and increased -. Human Cys-loop neurotransmitter receptor M3-M4 loops all exceed 70 residues. Conclusion: M3-M4 lengths of >70 are critical. Significance: Cytoplasmic portals may be essential for normal function of Cys-loop neurotransmitter receptors.",
    author = "Baptista-Hon, {Daniel T.} and Deeb, {Tarek Z.} and Lambert, {Jeremy J.} and Peters, {John A.} and Hales, {Tim G.}",
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    T1 - The minimum M3-M4 loop length of neurotransmitteractivated pentameric receptors is critical for the structural integrity of cytoplasmic portals

    AU - Baptista-Hon, Daniel T.

    AU - Deeb, Tarek Z.

    AU - Lambert, Jeremy J.

    AU - Peters, John A.

    AU - Hales, Tim G.

    PY - 2013/7/26

    Y1 - 2013/7/26

    N2 - Background: Residue 436 within 5-HT3A cytoplasmic portals determines single channel conductance (?) and rectification. Results: M3-M4 loop truncation to 75 residues spared inward rectification; truncation to 70 abolished rectification and increased -. Human Cys-loop neurotransmitter receptor M3-M4 loops all exceed 70 residues. Conclusion: M3-M4 lengths of >70 are critical. Significance: Cytoplasmic portals may be essential for normal function of Cys-loop neurotransmitter receptors.

    AB - Background: Residue 436 within 5-HT3A cytoplasmic portals determines single channel conductance (?) and rectification. Results: M3-M4 loop truncation to 75 residues spared inward rectification; truncation to 70 abolished rectification and increased -. Human Cys-loop neurotransmitter receptor M3-M4 loops all exceed 70 residues. Conclusion: M3-M4 lengths of >70 are critical. Significance: Cytoplasmic portals may be essential for normal function of Cys-loop neurotransmitter receptors.

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    JF - Journal of Biological Chemistry

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