The minimum M3-M4 loop length of neurotransmitteractivated pentameric receptors is critical for the structural integrity of cytoplasmic portals

Daniel T. Baptista-Hon; Tarek Z. Deeb; Jeremy J. Lambert; John A. Peters; Tim G. Hales

doi:10.1074/jbc.M113.481689

The minimum M3-M4 loop length of neurotransmitteractivated pentameric receptors is critical for the structural integrity of cytoplasmic portals

Daniel T. Baptista-Hon, Tarek Z. Deeb, Jeremy J. Lambert, John A. Peters, Tim G. Hales (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Background: Residue 436 within 5-HT3A cytoplasmic portals determines single channel conductance (?) and rectification. Results: M3-M4 loop truncation to 75 residues spared inward rectification; truncation to 70 abolished rectification and increased -. Human Cys-loop neurotransmitter receptor M3-M4 loops all exceed 70 residues. Conclusion: M3-M4 lengths of >70 are critical. Significance: Cytoplasmic portals may be essential for normal function of Cys-loop neurotransmitter receptors.

Original language	English
Pages (from-to)	21558-21568
Number of pages	11
Journal	Journal of Biological Chemistry
Volume	288
Issue number	30
DOIs	https://doi.org/10.1074/jbc.M113.481689
Publication status	Published - 26 Jul 2013

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abstract = "Background: Residue 436 within 5-HT3A cytoplasmic portals determines single channel conductance (?) and rectification. Results: M3-M4 loop truncation to 75 residues spared inward rectification; truncation to 70 abolished rectification and increased -. Human Cys-loop neurotransmitter receptor M3-M4 loops all exceed 70 residues. Conclusion: M3-M4 lengths of >70 are critical. Significance: Cytoplasmic portals may be essential for normal function of Cys-loop neurotransmitter receptors.",

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AU - Peters, John A.

AU - Hales, Tim G.

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AB - Background: Residue 436 within 5-HT3A cytoplasmic portals determines single channel conductance (?) and rectification. Results: M3-M4 loop truncation to 75 residues spared inward rectification; truncation to 70 abolished rectification and increased -. Human Cys-loop neurotransmitter receptor M3-M4 loops all exceed 70 residues. Conclusion: M3-M4 lengths of >70 are critical. Significance: Cytoplasmic portals may be essential for normal function of Cys-loop neurotransmitter receptors.

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The minimum M3-M4 loop length of neurotransmitteractivated pentameric receptors is critical for the structural integrity of cytoplasmic portals

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