The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation

Sara Carvalhal, Susana Abreu Ribeiro, Miguel Arocena, Taciana Kasciukovic, Achim Temme, Katrin Koehler, Angela Huebner, Eric R. Griffis (Lead / Corresponding author)

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Abstract

The formation of the mitotic spindle is a complex process that requires massive cellular reorganization. Regulation by mitotic kinases controls this entire process. One of these mitotic controllers is Aurora A kinase, which is itself highly regulated. In this study we show that the nuclear pore protein 2

ALADIN is a novel spatial regulator of Aurora A. Without ALADIN, we observe Aurora A spreading from centrosomes onto spindle microtubules, which affects the distribution of a subset of microtubule regulators and slows spindle assembly and chromosome alignment. ALADIN interacts with inactive Aurora A and is recruited to the spindle pole after Aurora A inhibition. Curiously, mutations in ALADIN cause triple A syndrome. We find some of the mitotic phenotypes that we observe after ALADIN depletion also occur in cells from triple A syndrome patients, which raises the possibility that mitotic errors may underlie the etiology of this syndrome.
Original languageEnglish
Pages (from-to)3424-3438
Number of pages15
JournalMolecular Biology of the Cell
Volume26
Issue number19
Early online date5 Aug 2015
DOIs
Publication statusPublished - 1 Oct 2015

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Carvalhal, S., Abreu Ribeiro, S., Arocena, M., Kasciukovic, T., Temme, A., Koehler, K., Huebner, A., & Griffis, E. R. (2015). The nucleoporin ALADIN regulates Aurora A localization to ensure robust mitotic spindle formation. Molecular Biology of the Cell, 26(19), 3424-3438. https://doi.org/10.1091/mbc.E15-02-0113