The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex

Melpomeni Platani (Lead / Corresponding author), Rachel Santarella-Mellwig, Markus Posch, Rudolf Walczak, Jason R. Swedlow, Iain W. Mattaj

    Research output: Contribution to journalArticle

    54 Citations (Scopus)

    Abstract

    The human Nup107-160 nucleoporin complex plays a major role in formation of the nuclear pore complex and is localized to kinetochores in mitosis. Here we report that Seh1, a component of the Nup107-160 complex, functions in chromosome alignment and segregation by regulating the centromeric localization of Aurora B and other chromosome passenger complex proteins. Localization of CENP-E is not affected by Seh1 depletion and analysis by electron microscopy showed that microtubule kinetochore attachments are intact. Seh1-depleted cells show impaired Aurora B localization, which results in severe defects in biorientation and organization of the spindle midzone and midbody. Our results indicate that a major function of the Nup107 complex in mitosis is to ensure the proper localization of the CPC at the centromere.

    Original languageEnglish
    Pages (from-to)5260-5275
    Number of pages16
    JournalMolecular Biology of the Cell
    Volume20
    Issue number24
    DOIs
    Publication statusPublished - 15 Dec 2009

    Keywords

    • NUCLEAR-PORE COMPLEX
    • AURORA-B KINASE
    • KINETOCHORE-MICROTUBULE ATTACHMENT
    • SPINDLE CHECKPOINT
    • CENP-E
    • SURVIVIN FUNCTION
    • CENTROMERIC MCAK
    • MAMMALIAN-CELLS
    • REGULATES MCAK
    • SOMATIC-CELLS

    Cite this

    Platani, Melpomeni ; Santarella-Mellwig, Rachel ; Posch, Markus ; Walczak, Rudolf ; Swedlow, Jason R. ; Mattaj, Iain W. / The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 24. pp. 5260-5275.
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    The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex. / Platani, Melpomeni (Lead / Corresponding author); Santarella-Mellwig, Rachel; Posch, Markus; Walczak, Rudolf; Swedlow, Jason R.; Mattaj, Iain W.

    In: Molecular Biology of the Cell, Vol. 20, No. 24, 15.12.2009, p. 5260-5275.

    Research output: Contribution to journalArticle

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    AU - Swedlow, Jason R.

    AU - Mattaj, Iain W.

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