The Nup107-160 nucleoporin complex promotes mitotic events via control of the localization state of the chromosome passenger complex

Melpomeni Platani (Lead / Corresponding author), Rachel Santarella-Mellwig, Markus Posch, Rudolf Walczak, Jason R. Swedlow, Iain W. Mattaj

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    Abstract

    The human Nup107-160 nucleoporin complex plays a major role in formation of the nuclear pore complex and is localized to kinetochores in mitosis. Here we report that Seh1, a component of the Nup107-160 complex, functions in chromosome alignment and segregation by regulating the centromeric localization of Aurora B and other chromosome passenger complex proteins. Localization of CENP-E is not affected by Seh1 depletion and analysis by electron microscopy showed that microtubule kinetochore attachments are intact. Seh1-depleted cells show impaired Aurora B localization, which results in severe defects in biorientation and organization of the spindle midzone and midbody. Our results indicate that a major function of the Nup107 complex in mitosis is to ensure the proper localization of the CPC at the centromere.

    Original languageEnglish
    Pages (from-to)5260-5275
    Number of pages16
    JournalMolecular Biology of the Cell
    Volume20
    Issue number24
    DOIs
    Publication statusPublished - 15 Dec 2009

    Keywords

    • NUCLEAR-PORE COMPLEX
    • AURORA-B KINASE
    • KINETOCHORE-MICROTUBULE ATTACHMENT
    • SPINDLE CHECKPOINT
    • CENP-E
    • SURVIVIN FUNCTION
    • CENTROMERIC MCAK
    • MAMMALIAN-CELLS
    • REGULATES MCAK
    • SOMATIC-CELLS

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