TY - JOUR
T1 - The orientation of the neuronal growth process can be directed via magnetic nanoparticles under an applied magnetic field
AU - Riggio, Cristina
AU - Calatayud, M. Pilar
AU - Giannaccini, Martina
AU - Sanz, Beatriz
AU - Torres, Teobaldo E.
AU - Fernández-Pacheco, Rodrigo
AU - Ripoli, Andrea
AU - Ibarra, Manuel Ricardo
AU - Dente, Luciana
AU - Cuschieri, Alfred
AU - Goya, Gerardo F.
AU - Raffa, Vittoria
N1 - Publisher Copyright:
© 2014 Elsevier Inc..
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - There is a growing body of evidence indicating the importance of physical stimuli for neuronal growth and development. Specifically, results from published experimental studies indicate that forces, when carefully controlled, can modulate neuronal regeneration. Here, we validate a non-invasive approach for physical guidance of nerve regeneration based on the synergic use of magnetic nanoparticles (MNPs) and magnetic fields (Ms). The concept is that the application of a tensile force to a neuronal cell can stimulate neurite initiation or axon elongation in the desired direction, the MNPs being used to generate this tensile force under the effect of a static external magnetic field providing the required directional orientation. In a neuron-like cell line, we have confirmed that MNPs direct the neurite outgrowth preferentially along the direction imposed by an external magnetic field, by inducing a net angle displacement (about 30°) of neurite direction. From the Clinical Editor: This study validates that non-invasive approaches for physical guidance of nerve regeneration based on the synergic use of magnetic nanoparticles and magnetic fields are possible. The hypothesis was confirmed by observing preferential neurite outgrowth in a cell culture system along the direction imposed by an external magnetic field.
AB - There is a growing body of evidence indicating the importance of physical stimuli for neuronal growth and development. Specifically, results from published experimental studies indicate that forces, when carefully controlled, can modulate neuronal regeneration. Here, we validate a non-invasive approach for physical guidance of nerve regeneration based on the synergic use of magnetic nanoparticles (MNPs) and magnetic fields (Ms). The concept is that the application of a tensile force to a neuronal cell can stimulate neurite initiation or axon elongation in the desired direction, the MNPs being used to generate this tensile force under the effect of a static external magnetic field providing the required directional orientation. In a neuron-like cell line, we have confirmed that MNPs direct the neurite outgrowth preferentially along the direction imposed by an external magnetic field, by inducing a net angle displacement (about 30°) of neurite direction. From the Clinical Editor: This study validates that non-invasive approaches for physical guidance of nerve regeneration based on the synergic use of magnetic nanoparticles and magnetic fields are possible. The hypothesis was confirmed by observing preferential neurite outgrowth in a cell culture system along the direction imposed by an external magnetic field.
KW - Magnetic field
KW - Magnetic nanoparticle
KW - Nerve regeneration
KW - Neurite outgrowth orientation
KW - Physical guidance
UR - http://www.scopus.com/inward/record.url?scp=84912151105&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2013.12.008
DO - 10.1016/j.nano.2013.12.008
M3 - Article
C2 - 24407149
AN - SCOPUS:84912151105
SN - 1549-9634
VL - 10
SP - 1549
EP - 1558
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 7
ER -