The osmotic responses of Penicillium ochro-chloron: Changes in internal solute levels in response to copper and salt stress

G. M. Gadd; J. A. Chudek; R. Foster; R. H. Reed

doi:10.1099/00221287-130-8-1969

The osmotic responses of Penicillium ochro-chloron: Changes in internal solute levels in response to copper and salt stress

G. M. Gadd
, J. A. Chudek
, R. Foster
, R. H. Reed

University of Dundee

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

The marked copper tolerance of Penicillium ochro-chloron has been confirmed as has its ability to grow in solutions of high salinity. The major low molecular weight organic solutes present in P. ochro-chloron were glycerol, erythritol, arabitol, mannitol, sorbitol and trehalose. Of these, glycerol increased significantly during growth in high concentrations of Na⁺ or Cu²⁺, a 15-fold increase, relative to the control, occurring in concentrations of 0.5 M. Cell K⁺ increased with elevated external Na⁺ but decreased slightly with elevated external Cu²⁺. With high external Cu²⁺ mycelium maintained a constant level of Cu²⁺, with low levels of Na⁺ in all treatments. It is concluded that the high concentrations of glycerol induced by high external Na⁺ or Cu²⁺ had a significant osmotic effect, allowing growth in media of high osmolality. The exclusion of ions may have a gratuitous role in the copper tolerance of P. ochro-chloron.

Original language	English
Pages (from-to)	1969-1975
Number of pages	7
Journal	Journal of General Microbiology
Volume	130
Issue number	8
DOIs	https://doi.org/10.1099/00221287-130-8-1969
Publication status	Published - 1 Aug 1984

ASJC Scopus subject areas

Microbiology

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10.1099/00221287-130-8-1969

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title = "The osmotic responses of Penicillium ochro-chloron: Changes in internal solute levels in response to copper and salt stress",

abstract = "The marked copper tolerance of Penicillium ochro-chloron has been confirmed as has its ability to grow in solutions of high salinity. The major low molecular weight organic solutes present in P. ochro-chloron were glycerol, erythritol, arabitol, mannitol, sorbitol and trehalose. Of these, glycerol increased significantly during growth in high concentrations of Na+ or Cu2+, a 15-fold increase, relative to the control, occurring in concentrations of 0.5 M. Cell K+ increased with elevated external Na+ but decreased slightly with elevated external Cu2+. With high external Cu2+ mycelium maintained a constant level of Cu2+, with low levels of Na+ in all treatments. It is concluded that the high concentrations of glycerol induced by high external Na+ or Cu2+ had a significant osmotic effect, allowing growth in media of high osmolality. The exclusion of ions may have a gratuitous role in the copper tolerance of P. ochro-chloron.",

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T1 - The osmotic responses of Penicillium ochro-chloron

T2 - Changes in internal solute levels in response to copper and salt stress

AU - Gadd, G. M.

AU - Chudek, J. A.

AU - Foster, R.

AU - Reed, R. H.

PY - 1984/8/1

Y1 - 1984/8/1

N2 - The marked copper tolerance of Penicillium ochro-chloron has been confirmed as has its ability to grow in solutions of high salinity. The major low molecular weight organic solutes present in P. ochro-chloron were glycerol, erythritol, arabitol, mannitol, sorbitol and trehalose. Of these, glycerol increased significantly during growth in high concentrations of Na+ or Cu2+, a 15-fold increase, relative to the control, occurring in concentrations of 0.5 M. Cell K+ increased with elevated external Na+ but decreased slightly with elevated external Cu2+. With high external Cu2+ mycelium maintained a constant level of Cu2+, with low levels of Na+ in all treatments. It is concluded that the high concentrations of glycerol induced by high external Na+ or Cu2+ had a significant osmotic effect, allowing growth in media of high osmolality. The exclusion of ions may have a gratuitous role in the copper tolerance of P. ochro-chloron.

AB - The marked copper tolerance of Penicillium ochro-chloron has been confirmed as has its ability to grow in solutions of high salinity. The major low molecular weight organic solutes present in P. ochro-chloron were glycerol, erythritol, arabitol, mannitol, sorbitol and trehalose. Of these, glycerol increased significantly during growth in high concentrations of Na+ or Cu2+, a 15-fold increase, relative to the control, occurring in concentrations of 0.5 M. Cell K+ increased with elevated external Na+ but decreased slightly with elevated external Cu2+. With high external Cu2+ mycelium maintained a constant level of Cu2+, with low levels of Na+ in all treatments. It is concluded that the high concentrations of glycerol induced by high external Na+ or Cu2+ had a significant osmotic effect, allowing growth in media of high osmolality. The exclusion of ions may have a gratuitous role in the copper tolerance of P. ochro-chloron.

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The osmotic responses of Penicillium ochro-chloron: Changes in internal solute levels in response to copper and salt stress

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