The osmotic responses of Saccharomyces cerevisiae in K+-depleted medium

Amanda J. Meikle; Robert H. Reed; Geoffrey M. Gadd

doi:10.1111/j.1574-6968.1991.tb04423.x

The osmotic responses of Saccharomyces cerevisiae in K⁺-depleted medium

Amanda J. Meikle
, Robert H. Reed
, Geoffrey M. Gadd (Lead / Corresponding author)

University of Dundee

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Saccharomyces cerevisiae showed reduced growth in a K⁺-depleted medium but was still capable of synthesizing and accumulating glycerol in the presence of 2.28% (w/v) NaCl; glycerol levels were similar in both K⁺-replete and K⁺-depleted media. The activity of glycerol-3-phosphate dehydrogenase, a key enzyme of glycerol synthesis, was inhibited to a similar extent by exogenous K⁺ and Na⁺ although such effects may be modified in vivo due to ionic compartmentation within the vacuole. These results indicate that exogenous K⁺ does not play a significant role in the initiation of glycerol synthesis in osmotically-stressed yeast.

Original language	English
Pages (from-to)	89-94
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	78
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04423.x
Publication status	Published - 1 Feb 1991

Keywords

Glycerol
Glycerol-3-phosphate dehydrogenase
Potassium
Saccharomyces cerevisiae

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1111/j.1574-6968.1991.tb04423.x

Cite this

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title = "The osmotic responses of Saccharomyces cerevisiae in K+-depleted medium",

abstract = "Saccharomyces cerevisiae showed reduced growth in a K+-depleted medium but was still capable of synthesizing and accumulating glycerol in the presence of 2.28\% (w/v) NaCl; glycerol levels were similar in both K+-replete and K+-depleted media. The activity of glycerol-3-phosphate dehydrogenase, a key enzyme of glycerol synthesis, was inhibited to a similar extent by exogenous K+ and Na+ although such effects may be modified in vivo due to ionic compartmentation within the vacuole. These results indicate that exogenous K+ does not play a significant role in the initiation of glycerol synthesis in osmotically-stressed yeast.",

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AU - Reed, Robert H.

AU - Gadd, Geoffrey M.

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N2 - Saccharomyces cerevisiae showed reduced growth in a K+-depleted medium but was still capable of synthesizing and accumulating glycerol in the presence of 2.28% (w/v) NaCl; glycerol levels were similar in both K+-replete and K+-depleted media. The activity of glycerol-3-phosphate dehydrogenase, a key enzyme of glycerol synthesis, was inhibited to a similar extent by exogenous K+ and Na+ although such effects may be modified in vivo due to ionic compartmentation within the vacuole. These results indicate that exogenous K+ does not play a significant role in the initiation of glycerol synthesis in osmotically-stressed yeast.

AB - Saccharomyces cerevisiae showed reduced growth in a K+-depleted medium but was still capable of synthesizing and accumulating glycerol in the presence of 2.28% (w/v) NaCl; glycerol levels were similar in both K+-replete and K+-depleted media. The activity of glycerol-3-phosphate dehydrogenase, a key enzyme of glycerol synthesis, was inhibited to a similar extent by exogenous K+ and Na+ although such effects may be modified in vivo due to ionic compartmentation within the vacuole. These results indicate that exogenous K+ does not play a significant role in the initiation of glycerol synthesis in osmotically-stressed yeast.

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KW - Glycerol-3-phosphate dehydrogenase

KW - Potassium

KW - Saccharomyces cerevisiae

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