The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4

Aleksandra A. Watson, Pravin Mahajan, Haydyn D. T. Mertens, M.J. Deery, Wanchao Zhang, Y. Chen, Christian Edlich, E.D. Laue, G. Berridge, Nicola A. Burgess-Brown, O. Gileadi, D.I. Svergun, P. Pham, Xiuxia Du, T. Bartke, T. Kouzarides, Nicola Wiechens, Tom Owen-Hughes

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    The NuRD (nucleosome remodeling and deacetylase) complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The core nucleosome remodeling function of the mammalian NuRD complex is executed by the helicase-domain-containing ATPase CHD4 (Mi-2ß) subunit, which also contains N-terminal plant homeodomain (PHD) and chromo domains. The mode of regulation of chromatin remodeling by CHD4 is not well understood, nor is the role of its PHD and chromo domains. Here, we use small-angle X-ray scattering, nucleosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spectrometry to propose a three-dimensional structural model describing the overall shape and domain interactions of CHD4 and discuss the relevance of these for regulating the remodeling of chromatin by the NuRD complex.
    Original languageEnglish
    Pages (from-to)3-17
    Number of pages15
    JournalJournal of Molecular Biology
    Volume422
    Issue number1
    DOIs
    Publication statusPublished - 2012

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    Mi-2 Nucleosome Remodeling and Deacetylase Complex
    Nucleosomes
    Human Activities
    Chromatin
    Adenosine Triphosphatases
    Chromatin Assembly and Disassembly
    Structural Models
    Tandem Mass Spectrometry
    Epigenomics
    Histones
    Proteolysis
    Cell Differentiation
    Transcription Factors
    Cell Proliferation
    X-Rays

    Cite this

    Watson, Aleksandra A. ; Mahajan, Pravin ; Mertens, Haydyn D. T. ; Deery, M.J. ; Zhang, Wanchao ; Chen, Y. ; Edlich, Christian ; Laue, E.D. ; Berridge, G. ; Burgess-Brown, Nicola A. ; Gileadi, O. ; Svergun, D.I. ; Pham, P. ; Du, Xiuxia ; Bartke, T. ; Kouzarides, T. ; Wiechens, Nicola ; Owen-Hughes, Tom. / The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4. In: Journal of Molecular Biology. 2012 ; Vol. 422, No. 1. pp. 3-17.
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    title = "The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4",
    abstract = "The NuRD (nucleosome remodeling and deacetylase) complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The core nucleosome remodeling function of the mammalian NuRD complex is executed by the helicase-domain-containing ATPase CHD4 (Mi-2{\ss}) subunit, which also contains N-terminal plant homeodomain (PHD) and chromo domains. The mode of regulation of chromatin remodeling by CHD4 is not well understood, nor is the role of its PHD and chromo domains. Here, we use small-angle X-ray scattering, nucleosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spectrometry to propose a three-dimensional structural model describing the overall shape and domain interactions of CHD4 and discuss the relevance of these for regulating the remodeling of chromatin by the NuRD complex.",
    author = "Watson, {Aleksandra A.} and Pravin Mahajan and Mertens, {Haydyn D. T.} and M.J. Deery and Wanchao Zhang and Y. Chen and Christian Edlich and E.D. Laue and G. Berridge and Burgess-Brown, {Nicola A.} and O. Gileadi and D.I. Svergun and P. Pham and Xiuxia Du and T. Bartke and T. Kouzarides and Nicola Wiechens and Tom Owen-Hughes",
    note = "Copyright 2012 Elsevier B.V., All rights reserved.",
    year = "2012",
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    language = "English",
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    Watson, AA, Mahajan, P, Mertens, HDT, Deery, MJ, Zhang, W, Chen, Y, Edlich, C, Laue, ED, Berridge, G, Burgess-Brown, NA, Gileadi, O, Svergun, DI, Pham, P, Du, X, Bartke, T, Kouzarides, T, Wiechens, N & Owen-Hughes, T 2012, 'The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4', Journal of Molecular Biology, vol. 422, no. 1, pp. 3-17. https://doi.org/10.1016/j.jmb.2012.04.031

    The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4. / Watson, Aleksandra A. ; Mahajan, Pravin; Mertens, Haydyn D. T.; Deery, M.J.; Zhang, Wanchao; Chen, Y.; Edlich, Christian; Laue, E.D.; Berridge, G.; Burgess-Brown, Nicola A.; Gileadi, O.; Svergun, D.I.; Pham, P.; Du, Xiuxia; Bartke, T.; Kouzarides, T.; Wiechens, Nicola; Owen-Hughes, Tom.

    In: Journal of Molecular Biology, Vol. 422, No. 1, 2012, p. 3-17.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4

    AU - Watson, Aleksandra A.

    AU - Mahajan, Pravin

    AU - Mertens, Haydyn D. T.

    AU - Deery, M.J.

    AU - Zhang, Wanchao

    AU - Chen, Y.

    AU - Edlich, Christian

    AU - Laue, E.D.

    AU - Berridge, G.

    AU - Burgess-Brown, Nicola A.

    AU - Gileadi, O.

    AU - Svergun, D.I.

    AU - Pham, P.

    AU - Du, Xiuxia

    AU - Bartke, T.

    AU - Kouzarides, T.

    AU - Wiechens, Nicola

    AU - Owen-Hughes, Tom

    N1 - Copyright 2012 Elsevier B.V., All rights reserved.

    PY - 2012

    Y1 - 2012

    N2 - The NuRD (nucleosome remodeling and deacetylase) complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The core nucleosome remodeling function of the mammalian NuRD complex is executed by the helicase-domain-containing ATPase CHD4 (Mi-2ß) subunit, which also contains N-terminal plant homeodomain (PHD) and chromo domains. The mode of regulation of chromatin remodeling by CHD4 is not well understood, nor is the role of its PHD and chromo domains. Here, we use small-angle X-ray scattering, nucleosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spectrometry to propose a three-dimensional structural model describing the overall shape and domain interactions of CHD4 and discuss the relevance of these for regulating the remodeling of chromatin by the NuRD complex.

    AB - The NuRD (nucleosome remodeling and deacetylase) complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The core nucleosome remodeling function of the mammalian NuRD complex is executed by the helicase-domain-containing ATPase CHD4 (Mi-2ß) subunit, which also contains N-terminal plant homeodomain (PHD) and chromo domains. The mode of regulation of chromatin remodeling by CHD4 is not well understood, nor is the role of its PHD and chromo domains. Here, we use small-angle X-ray scattering, nucleosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spectrometry to propose a three-dimensional structural model describing the overall shape and domain interactions of CHD4 and discuss the relevance of these for regulating the remodeling of chromatin by the NuRD complex.

    UR - http://www.scopus.com/inward/record.url?scp=84861332377&partnerID=8YFLogxK

    U2 - 10.1016/j.jmb.2012.04.031

    DO - 10.1016/j.jmb.2012.04.031

    M3 - Article

    VL - 422

    SP - 3

    EP - 17

    JO - Journal of Molecular Biology

    JF - Journal of Molecular Biology

    SN - 0022-2836

    IS - 1

    ER -