Projects per year
Abstract
The k-turn is a widespread structural motif that introduces a tight kink into the helical axis of double-stranded RNA. The adenine bases of consecutive G.A pairs are directed toward the minor groove of the opposing helix, hydrogen bonding in a typical A-minor interaction. We show here that the available structures of k-turns divide into two classes, depending on whether N3 or N1 of the adenine at the 2b position accepts a hydrogen bond from the O2' at the -1n position. There is a coordinated structural change involving a number of hydrogen bonds between the two classes. We show here that Kt-7 can adopt either the N3 or N1 structures depending on environment. While it has the N1 structure in the ribosome, on engineering it into the SAM-I riboswitch, it changes to the N3 structure, resulting in a significant alteration in the trajectory of the helical arms.
Original language | English |
---|---|
Pages (from-to) | 357-364 |
Number of pages | 8 |
Journal | RNA: a Publication of the RNA Society |
Volume | 19 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- SEQUENCE
- BINDING
- SAM-I riboswitch
- ANGSTROM RESOLUTION
- PROTEIN
- LARGE RIBOSOMAL-SUBUNIT
- k-turn
- RNA structure
- COMPLEX
- RNA-protein interaction
- L7AE
- APTAMER
- TERTIARY INTERACTIONS
- RIBOSWITCH
Fingerprint
Dive into the research topics of 'The plasticity of a structural motif in RNA: Structural polymorphism of a kink turn as a function of its environment'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Fluorescence Resonance Energy Transfer as a Rich Source of Orientational Information in Nucleic Acid Structure
Lilley, D. (Investigator)
Engineering and Physical Sciences Research Council
1/09/12 → 30/06/16
Project: Research