The potent osteo‑inductive capacity of bioinspired brown seaweed‑derived carbohydrate nanofbrous three‑dimensional scafolds

TY - JOUR

T1 - The potent osteo‑inductive capacity of bioinspired brown seaweed‑derived carbohydrate nanofbrous three‑dimensional scafolds

AU - Ouranj, Zahra Dadashi

AU - Hosseini, Saadi

AU - Alipour, Atefeh

AU - Homaeigohar, Shahin

AU - Azari, Shahram

AU - Ghazizadeh, Leila

AU - Shokrgozar, Mohammadali

AU - Thomas, Sabu

AU - Irian, Saeed

AU - Shahsavarani, Hosein

N1 - Copyright: © 2024, Ocean University of China.

PY - 2024/7/24

Y1 - 2024/7/24

N2 - This study aimed to investigate the osteo-inductive capacity of a fucoidan polysaccharide network derived from brown algaeon human adipose-derived stem cells (HA-MSCs) for bone regeneration. The physiochemical properties of the scafoldincluding surface morphology, surface chemistry, hydrophilicity, mechanical stifness, and porosity were thoroughly characterized. Both in vitro and in vivo measurements implied a superior cell viability, proliferation, adhesion, and osteo-inductiveperformance of obtained scafolds compared to using specifc osteogenic induction medium with increased irregular growthof calcium crystallites, which mimic the structure of natural bones. That scafold was highly biocompatible and suitable forcell cultures. Various examinations, such as quantifcation of mineralization, alkaline phosphatase, gene expression, andimmunocytochemical staining of pre-osteocyte and bone markers confrmed that HAD-MSCs diferentiate into osteoblasts,even without an osteogenic induction medium. This study provides evidence for the positive relationship and synergisticefects between the physical properties of the decellularized seaweed scafold and the chemical composition of fucoidan inpromoting the osteogenic diferentiation of HA-MSCs. Altogether, the natural matrices derived from brown seaweed ofersa sustainable, cost-efective, non-toxic bioinspired scafold and holds promise for future clinical applications in orthopedics.

AB - This study aimed to investigate the osteo-inductive capacity of a fucoidan polysaccharide network derived from brown algaeon human adipose-derived stem cells (HA-MSCs) for bone regeneration. The physiochemical properties of the scafoldincluding surface morphology, surface chemistry, hydrophilicity, mechanical stifness, and porosity were thoroughly characterized. Both in vitro and in vivo measurements implied a superior cell viability, proliferation, adhesion, and osteo-inductiveperformance of obtained scafolds compared to using specifc osteogenic induction medium with increased irregular growthof calcium crystallites, which mimic the structure of natural bones. That scafold was highly biocompatible and suitable forcell cultures. Various examinations, such as quantifcation of mineralization, alkaline phosphatase, gene expression, andimmunocytochemical staining of pre-osteocyte and bone markers confrmed that HAD-MSCs diferentiate into osteoblasts,even without an osteogenic induction medium. This study provides evidence for the positive relationship and synergisticefects between the physical properties of the decellularized seaweed scafold and the chemical composition of fucoidan inpromoting the osteogenic diferentiation of HA-MSCs. Altogether, the natural matrices derived from brown seaweed ofersa sustainable, cost-efective, non-toxic bioinspired scafold and holds promise for future clinical applications in orthopedics.

KW - Bone tissue engineering

KW - Seaweed-derived scafold

KW - Fucoidans

KW - HA-MSCs diferentiation

KW - Osteoblasts

KW - Seaweed-derived scaffold

KW - HA-MSCs differentiation

UR - https://www.scopus.com/pages/publications/85199337452

U2 - 10.1007/s42995-024-00241-1

DO - 10.1007/s42995-024-00241-1

M3 - Article

C2 - 39219680

SN - 2096-6490

VL - 6

SP - 515

EP - 534

JO - Marine Life Science & Technology

JF - Marine Life Science & Technology

IS - 3

ER -