The reduction of polyhalogenated methanes by liver microsomal cytochrome P450

C. R. Wolf, D. Mansuy, W. Nastainczyk, G. Deutschmann, V. Ullrich

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Abstract

Under anaerobic reducing conditions various polyhalogenated methanes (CBr 4, CCl 4, CCl 3F, CCl 3Br, CCl 3CN, CHI 3, CHBr 3, and CHCl 3) form complexes with ferrous cytochrome P450 with absorption peaks in the difference spectra ranging between 450 and 470 nm. Carbon monoxide was detected as a metabolic product of the interaction. Inhibition experiments indicated the involvement of cytochrome P450 in the reduction process, and a reaction sequence is proposed to account for these findings. Comparison of cytochrome P450 complex formation using liver microsomal preparations from phenobarbital- and 3,4 benzpyrene-treated rats showed differences which could be accounted for by decreased stability of the halogenomethane complex with the 3,4 benzpyrene-induced form of cytochrome P450.

Original languageEnglish
Pages (from-to)698-705
Number of pages8
JournalMolecular Pharmacology
Volume13
Issue number4
Publication statusPublished - 1 Dec 1977

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Methane
Cytochrome P-450 Enzyme System
Benzo(a)pyrene
Liver
Carbon Monoxide
Phenobarbital

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Wolf, C. R., Mansuy, D., Nastainczyk, W., Deutschmann, G., & Ullrich, V. (1977). The reduction of polyhalogenated methanes by liver microsomal cytochrome P450. Molecular Pharmacology, 13(4), 698-705.
Wolf, C. R. ; Mansuy, D. ; Nastainczyk, W. ; Deutschmann, G. ; Ullrich, V. / The reduction of polyhalogenated methanes by liver microsomal cytochrome P450. In: Molecular Pharmacology. 1977 ; Vol. 13, No. 4. pp. 698-705.
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Wolf, CR, Mansuy, D, Nastainczyk, W, Deutschmann, G & Ullrich, V 1977, 'The reduction of polyhalogenated methanes by liver microsomal cytochrome P450', Molecular Pharmacology, vol. 13, no. 4, pp. 698-705.

The reduction of polyhalogenated methanes by liver microsomal cytochrome P450. / Wolf, C. R.; Mansuy, D.; Nastainczyk, W.; Deutschmann, G.; Ullrich, V.

In: Molecular Pharmacology, Vol. 13, No. 4, 01.12.1977, p. 698-705.

Research output: Contribution to journalArticle

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AU - Ullrich, V.

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AB - Under anaerobic reducing conditions various polyhalogenated methanes (CBr 4, CCl 4, CCl 3F, CCl 3Br, CCl 3CN, CHI 3, CHBr 3, and CHCl 3) form complexes with ferrous cytochrome P450 with absorption peaks in the difference spectra ranging between 450 and 470 nm. Carbon monoxide was detected as a metabolic product of the interaction. Inhibition experiments indicated the involvement of cytochrome P450 in the reduction process, and a reaction sequence is proposed to account for these findings. Comparison of cytochrome P450 complex formation using liver microsomal preparations from phenobarbital- and 3,4 benzpyrene-treated rats showed differences which could be accounted for by decreased stability of the halogenomethane complex with the 3,4 benzpyrene-induced form of cytochrome P450.

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Wolf CR, Mansuy D, Nastainczyk W, Deutschmann G, Ullrich V. The reduction of polyhalogenated methanes by liver microsomal cytochrome P450. Molecular Pharmacology. 1977 Dec 1;13(4):698-705.