The reduction of polyhalogenated methanes by liver microsomal cytochrome P450

C. R. Wolf; D. Mansuy; W. Nastainczyk; G. Deutschmann; V. Ullrich

doi:10.1016/S0026-895X(25)12433-4

The reduction of polyhalogenated methanes by liver microsomal cytochrome P450

C. R. Wolf
, D. Mansuy
, W. Nastainczyk
, G. Deutschmann
, V. Ullrich

Research output: Contribution to journal › Article › peer-review

145 Citations (Scopus)

Abstract

Under anaerobic reducing conditions various polyhalogenated methanes (CBr ₄, CCl ₄, CCl ₃F, CCl ₃Br, CCl ₃CN, CHI ₃, CHBr ₃, and CHCl ₃) form complexes with ferrous cytochrome P450 with absorption peaks in the difference spectra ranging between 450 and 470 nm. Carbon monoxide was detected as a metabolic product of the interaction. Inhibition experiments indicated the involvement of cytochrome P450 in the reduction process, and a reaction sequence is proposed to account for these findings. Comparison of cytochrome P450 complex formation using liver microsomal preparations from phenobarbital- and 3,4 benzpyrene-treated rats showed differences which could be accounted for by decreased stability of the halogenomethane complex with the 3,4 benzpyrene-induced form of cytochrome P450.

Original language	English
Pages (from-to)	698-705
Number of pages	8
Journal	Molecular Pharmacology
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/S0026-895X(25)12433-4
Publication status	Published - 1 Dec 1977

ASJC Scopus subject areas

Pharmacology

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10.1016/S0026-895X(25)12433-4

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title = "The reduction of polyhalogenated methanes by liver microsomal cytochrome P450",

abstract = "Under anaerobic reducing conditions various polyhalogenated methanes (CBr 4, CCl 4, CCl 3F, CCl 3Br, CCl 3CN, CHI 3, CHBr 3, and CHCl 3) form complexes with ferrous cytochrome P450 with absorption peaks in the difference spectra ranging between 450 and 470 nm. Carbon monoxide was detected as a metabolic product of the interaction. Inhibition experiments indicated the involvement of cytochrome P450 in the reduction process, and a reaction sequence is proposed to account for these findings. Comparison of cytochrome P450 complex formation using liver microsomal preparations from phenobarbital- and 3,4 benzpyrene-treated rats showed differences which could be accounted for by decreased stability of the halogenomethane complex with the 3,4 benzpyrene-induced form of cytochrome P450.",

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T1 - The reduction of polyhalogenated methanes by liver microsomal cytochrome P450

AU - Wolf, C. R.

AU - Mansuy, D.

AU - Nastainczyk, W.

AU - Deutschmann, G.

AU - Ullrich, V.

PY - 1977/12/1

Y1 - 1977/12/1

N2 - Under anaerobic reducing conditions various polyhalogenated methanes (CBr 4, CCl 4, CCl 3F, CCl 3Br, CCl 3CN, CHI 3, CHBr 3, and CHCl 3) form complexes with ferrous cytochrome P450 with absorption peaks in the difference spectra ranging between 450 and 470 nm. Carbon monoxide was detected as a metabolic product of the interaction. Inhibition experiments indicated the involvement of cytochrome P450 in the reduction process, and a reaction sequence is proposed to account for these findings. Comparison of cytochrome P450 complex formation using liver microsomal preparations from phenobarbital- and 3,4 benzpyrene-treated rats showed differences which could be accounted for by decreased stability of the halogenomethane complex with the 3,4 benzpyrene-induced form of cytochrome P450.

AB - Under anaerobic reducing conditions various polyhalogenated methanes (CBr 4, CCl 4, CCl 3F, CCl 3Br, CCl 3CN, CHI 3, CHBr 3, and CHCl 3) form complexes with ferrous cytochrome P450 with absorption peaks in the difference spectra ranging between 450 and 470 nm. Carbon monoxide was detected as a metabolic product of the interaction. Inhibition experiments indicated the involvement of cytochrome P450 in the reduction process, and a reaction sequence is proposed to account for these findings. Comparison of cytochrome P450 complex formation using liver microsomal preparations from phenobarbital- and 3,4 benzpyrene-treated rats showed differences which could be accounted for by decreased stability of the halogenomethane complex with the 3,4 benzpyrene-induced form of cytochrome P450.

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DO - 10.1016/S0026-895X(25)12433-4

M3 - Article

C2 - 18662

AN - SCOPUS:0017654672

SN - 0026-895X

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JO - Molecular Pharmacology

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ER -

The reduction of polyhalogenated methanes by liver microsomal cytochrome P450

Abstract

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