The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coli

Vijaya Satchidanandam; Rama Rao Amara; Pradeep Devappa Uchil; Varsha Singh

doi:10.1016/S0378-1097(02)01185-0

The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coli

Vijaya Satchidanandam (Lead / Corresponding author)
, Rama Rao Amara
, Pradeep Devappa Uchil
, Varsha Singh

Molecular Microbiology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We report efficient expression of the Mycobacterium tuberculosis gene Rv3881c in Escherichia coli from its M. tuberculosis promoter, attributable to an E. coli consensus Pribnow box and ribosome binding site. The N-terminal sequence of the recombinant E. coli-generated protein was identical to the predicted open reading frame of Rv3881c and transcription of the Rv3881c gene initiated at the same nucleotide position in both bacteria. We demonstrate the utility of this promoter for rapid analysis of expression in E. coli of heterologous gene constructs, for subsequent expression from the genomes of slow-growing mycobacteria such as Mycobacterium bovis-BCG. M. tuberculosis Rv3881c homologues were present in other pathogenic mycobacteria such as M. bovis-BCG, Mycobacterium szulgai and Mycobacterium kansasii.

Original language	English
Pages (from-to)	365-370
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	218
Issue number	2
Early online date	25 Dec 2002
DOIs	https://doi.org/10.1016/S0378-1097(02)01185-0
Publication status	Published - 1 Jan 2003

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Mycobacterium tuberculosis
Promoter
Ribosome binding site
Rv3881c

ASJC Scopus subject areas

General Medicine

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10.1016/S0378-1097(02)01185-0

Cite this

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title = "The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coli",

abstract = "We report efficient expression of the Mycobacterium tuberculosis gene Rv3881c in Escherichia coli from its M. tuberculosis promoter, attributable to an E. coli consensus Pribnow box and ribosome binding site. The N-terminal sequence of the recombinant E. coli-generated protein was identical to the predicted open reading frame of Rv3881c and transcription of the Rv3881c gene initiated at the same nucleotide position in both bacteria. We demonstrate the utility of this promoter for rapid analysis of expression in E. coli of heterologous gene constructs, for subsequent expression from the genomes of slow-growing mycobacteria such as Mycobacterium bovis-BCG. M. tuberculosis Rv3881c homologues were present in other pathogenic mycobacteria such as M. bovis-BCG, Mycobacterium szulgai and Mycobacterium kansasii.",

keywords = "Mycobacterium tuberculosis, Promoter, Ribosome binding site, Rv3881c",

author = "Vijaya Satchidanandam and Amara, \{Rama Rao\} and Uchil, \{Pradeep Devappa\} and Varsha Singh",

note = "Copyright {\textcopyright} 2003, {\textcopyright} 2002 Federation of European Microbiological Societies",

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AU - Satchidanandam, Vijaya

AU - Amara, Rama Rao

AU - Uchil, Pradeep Devappa

AU - Singh, Varsha

PY - 2003/1/1

Y1 - 2003/1/1

N2 - We report efficient expression of the Mycobacterium tuberculosis gene Rv3881c in Escherichia coli from its M. tuberculosis promoter, attributable to an E. coli consensus Pribnow box and ribosome binding site. The N-terminal sequence of the recombinant E. coli-generated protein was identical to the predicted open reading frame of Rv3881c and transcription of the Rv3881c gene initiated at the same nucleotide position in both bacteria. We demonstrate the utility of this promoter for rapid analysis of expression in E. coli of heterologous gene constructs, for subsequent expression from the genomes of slow-growing mycobacteria such as Mycobacterium bovis-BCG. M. tuberculosis Rv3881c homologues were present in other pathogenic mycobacteria such as M. bovis-BCG, Mycobacterium szulgai and Mycobacterium kansasii.

AB - We report efficient expression of the Mycobacterium tuberculosis gene Rv3881c in Escherichia coli from its M. tuberculosis promoter, attributable to an E. coli consensus Pribnow box and ribosome binding site. The N-terminal sequence of the recombinant E. coli-generated protein was identical to the predicted open reading frame of Rv3881c and transcription of the Rv3881c gene initiated at the same nucleotide position in both bacteria. We demonstrate the utility of this promoter for rapid analysis of expression in E. coli of heterologous gene constructs, for subsequent expression from the genomes of slow-growing mycobacteria such as Mycobacterium bovis-BCG. M. tuberculosis Rv3881c homologues were present in other pathogenic mycobacteria such as M. bovis-BCG, Mycobacterium szulgai and Mycobacterium kansasii.

KW - Mycobacterium tuberculosis

KW - Promoter

KW - Ribosome binding site

KW - Rv3881c

UR - https://www.scopus.com/pages/publications/0037469274

U2 - 10.1016/S0378-1097(02)01185-0

DO - 10.1016/S0378-1097(02)01185-0

M3 - Article

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AN - SCOPUS:0037469274

SN - 0378-1097

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SP - 365

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JF - FEMS Microbiology Letters

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ER -

The regulatory elements of the Mycobacterium tuberculosis gene Rv3881c function efficiently in Escherichia coli

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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