The relationship of oxidative stress to thrombotic tendency in type 1 diabetic patients with retinopathy

P. E. Jennings, M. McLaren, N. A. Scott, A. R. Saniabadi, J. J. F. Belch

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    Abstract

    Increased free radical activity may contribute to thrombosis via effects on platelet aggregation and the prostanoid balance. To investigate this further we studied 15 Type 1 diabetic patients with retinopathy, matched with uncomplicated Type 1 patients for age, duration of diabetes and HbA1, together with matched healthy non-diabetic control subjects. The oxidative effects of free radicals as total diene conjugates and lipid peroxides were measured, together with redox status extracellularly as plasma albumin-thiols and intracellularly as erythrocyte superoxide dismutase activity. Platelet count, aggregation of platelets in whole blood to collagen, thromboxane B2, and prostacyclin stimulating factor (PGI2SF) were also assessed. Free radicals measured as lipid peroxides were significantly higher (9.6 (8.1-11.6) µmol 1-1 (median and interquartile range) in diabetic patients with retinopathy than in control subjects (8.1 (7.4-9.2) µmol 1-1; p < 0.05). There were also significant reductions in redox status both extracellularly as plasma albumin thiols (408 (383-473) vs 490 (456-517) µmol 1-1, p < 0.001) and intracellularly as erythrocyte superoxide dismutase activity (34 (27-41) vs 44 (36-51) g 1-1, p < 0.05) between patients with retinopathy and control subjects. Platelet counts were increased in diabetic patients with retinopathy (p < 0.05), as was collagen-induced platelet aggregation (p < 0.01). Prostacyclin stimulating factor was reduced in patients with retinopathy (p < 0.05) and correlated within the plasma with lipid peroxides (r = -0.53, p < 0.04) and albumin thiols (r = 0.64, p < 0.01). The results suggest that diabetic patients, particularly with retinopathy, are under oxidative stress and have an increased thrombotic tendency with increased platelet reactivity and a reduction in prostacyclin stimulating factor.

    Original languageEnglish
    Pages (from-to)860-865
    Number of pages6
    JournalDiabetic Medicine
    Volume8
    Issue number9
    DOIs
    Publication statusPublished - Nov 1991

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    Oxidative Stress
    Lipid Peroxides
    Epoprostenol
    Platelet Aggregation
    Sulfhydryl Compounds
    Free Radicals
    Platelet Count
    Serum Albumin
    Superoxide Dismutase
    Oxidation-Reduction
    Collagen
    Blood Platelets
    Erythrocytes
    Thromboxane B2
    Glycosylated Hemoglobin A
    Prostaglandins
    Albumins
    Thrombosis

    Cite this

    Jennings, P. E. ; McLaren, M. ; Scott, N. A. ; Saniabadi, A. R. ; Belch, J. J. F. / The relationship of oxidative stress to thrombotic tendency in type 1 diabetic patients with retinopathy. In: Diabetic Medicine. 1991 ; Vol. 8, No. 9. pp. 860-865.
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    abstract = "Increased free radical activity may contribute to thrombosis via effects on platelet aggregation and the prostanoid balance. To investigate this further we studied 15 Type 1 diabetic patients with retinopathy, matched with uncomplicated Type 1 patients for age, duration of diabetes and HbA1, together with matched healthy non-diabetic control subjects. The oxidative effects of free radicals as total diene conjugates and lipid peroxides were measured, together with redox status extracellularly as plasma albumin-thiols and intracellularly as erythrocyte superoxide dismutase activity. Platelet count, aggregation of platelets in whole blood to collagen, thromboxane B2, and prostacyclin stimulating factor (PGI2SF) were also assessed. Free radicals measured as lipid peroxides were significantly higher (9.6 (8.1-11.6) µmol 1-1 (median and interquartile range) in diabetic patients with retinopathy than in control subjects (8.1 (7.4-9.2) µmol 1-1; p < 0.05). There were also significant reductions in redox status both extracellularly as plasma albumin thiols (408 (383-473) vs 490 (456-517) µmol 1-1, p < 0.001) and intracellularly as erythrocyte superoxide dismutase activity (34 (27-41) vs 44 (36-51) g 1-1, p < 0.05) between patients with retinopathy and control subjects. Platelet counts were increased in diabetic patients with retinopathy (p < 0.05), as was collagen-induced platelet aggregation (p < 0.01). Prostacyclin stimulating factor was reduced in patients with retinopathy (p < 0.05) and correlated within the plasma with lipid peroxides (r = -0.53, p < 0.04) and albumin thiols (r = 0.64, p < 0.01). The results suggest that diabetic patients, particularly with retinopathy, are under oxidative stress and have an increased thrombotic tendency with increased platelet reactivity and a reduction in prostacyclin stimulating factor.",
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    The relationship of oxidative stress to thrombotic tendency in type 1 diabetic patients with retinopathy. / Jennings, P. E.; McLaren, M.; Scott, N. A.; Saniabadi, A. R.; Belch, J. J. F.

    In: Diabetic Medicine, Vol. 8, No. 9, 11.1991, p. 860-865.

    Research output: Contribution to journalArticle

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