The release of immunoreactive neuropeptide Y in the spinal cord of the anaesthetized rat and cat

M. A. Mark, B. Jarrott, L. A. Colvin, S. J. A. MacMillan, A. W. Duggan (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


The release of immunoreactive (ir-) neuropeptide Y (NYP) was studied in the anaesthetized rat and cat by means of microprobes bearing immobilized antibodies to the C terminus of NPY. An extensive basal release of ir-NYP was detected throughout the dorsal and upper ventral horn of the rat. This spontaneous release was not significantly altered by sectioning the spinal cord at the thoraco-lumbar junction nor by electrical stimulation of peripheral nerves. Since NPY is virtually absent in primary afferents it is probable that spontaneous release within the spinal cord comes from active NPY-containing intrinsic spinal neurones. In the spinal cat spontaneous release of ir-NPY was detected in the mid-dorsal horn and this was unaltered by peripheral noxious thermal or noxious mechanical stimuli. As in the rat, release from intrinsic spinal neurones is most probable. The extensive spontaneous release of ir-NPY in both species suggests a widespread role in spinal cord function.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalBrain Research
Issue number1-2
Publication statusPublished - 18 Apr 1997


  • antibody microprobe
  • cat spinal cord
  • neuropeptide Y
  • rat spinal cord
  • spinal transection
  • spontaneous release

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology


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