The RhoA transcriptional program in pre-T cells

M. Mullin, K. Lightfoot, R. Clarke, D. Cantrell, M. Miller, R. Lahesmaa

    Research output: Contribution to journalArticle

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    Abstract

    The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T cells because it is required for the expression of transcription factors of the Egr-1 and AP-1 families that have critical functions in thymocyte development. Loss of RhoA function in T cell progenitors causes a developmental block that pheno-copies the consequence of losing pre-TCR expression in Recombinase gene 2 (Rag2) null mice. Transcriptional profiling reveals both common and unique gene targets for RhoA and the pre-TCR indicating that RhoA participates in the pre-TCR induced transcriptional program but also mediates pre-TCR independent gene transcription.
    Original languageEnglish
    Pages (from-to)4309-4317
    Number of pages9
    JournalFEBS Letters
    Volume581
    Issue number22
    DOIs
    Publication statusPublished - 4 Sep 2007

    Fingerprint

    T-cells
    Genes
    T-Lymphocytes
    Thymocytes
    Thymus
    Recombinases
    GTP Phosphohydrolases
    Transcription Factor AP-1
    Transcription
    Thymus Gland
    Transcription Factors

    Cite this

    Mullin, M. ; Lightfoot, K. ; Clarke, R. ; Cantrell, D. ; Miller, M. ; Lahesmaa, R. / The RhoA transcriptional program in pre-T cells. In: FEBS Letters. 2007 ; Vol. 581, No. 22. pp. 4309-4317.
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    Mullin, M, Lightfoot, K, Clarke, R, Cantrell, D, Miller, M & Lahesmaa, R 2007, 'The RhoA transcriptional program in pre-T cells', FEBS Letters, vol. 581, no. 22, pp. 4309-4317. https://doi.org/10.1016/j.febslet.2007.07.077

    The RhoA transcriptional program in pre-T cells. / Mullin, M.; Lightfoot, K.; Clarke, R.; Cantrell, D.; Miller, M.; Lahesmaa, R.

    In: FEBS Letters, Vol. 581, No. 22, 04.09.2007, p. 4309-4317.

    Research output: Contribution to journalArticle

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