The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Guillaume Jannot; Sarah Bajan; Nellie J. Giguère; Samir Bouasker; Isabelle H. Banville; Sandra Piquet; Gyorgy Hutvagner; Martin J. Simard

doi:10.1038/embor.2011.66

The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Guillaume Jannot, Sarah Bajan, Nellie J. Giguère, Samir Bouasker, Isabelle H. Banville, Sandra Piquet, Gyorgy Hutvagner, Martin J. Simard

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Abstract

Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression.

Original language	English
Pages (from-to)	581-586
Number of pages	6
Journal	EMBO Reports
Volume	12
Issue number	6
DOIs	https://doi.org/10.1038/embor.2011.66
Publication status	Published - Jun 2011

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abstract = "Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression.",

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AU - Bajan, Sarah

AU - Giguère, Nellie J.

AU - Bouasker, Samir

AU - Banville, Isabelle H.

AU - Piquet, Sandra

AU - Hutvagner, Gyorgy

AU - Simard, Martin J.

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AB - Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex-or miRNA-induced silencing complex (miRISC)-can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression.

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The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

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