The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation

Rebecca Lyons, Akira Iwase, Thomas Gänsewig, Alexander Sherstnev, Céline Duc, Geoffrey J. Barton, Kousuke Hanada, Mieko Higuchi-Takeuchi, Minami Matsui, Keiko Sugimoto, Kemal Kazan, Gordon G. Simpson, Ken Shirasu

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    RNA-binding proteins (RBPs) play an important role in plant host-microbe interactions. In this study, we show that the plant RBP known as FPA, which regulates 3'-end mRNA polyadenylation, negatively regulates basal resistance to bacterial pathogen Pseudomonas syringae in Arabidopsis. A custom microarray analysis reveals that flg22, a peptide derived from bacterial flagellins, induces expression of alternatively polyadenylated isoforms of mRNA encoding the defence-related transcriptional repressor ETHYLENE RESPONSE FACTOR 4 (ERF4), which is regulated by FPA. Flg22 induces expression of a novel isoform of ERF4 that lacks the ERF-associated amphiphilic repression (EAR) motif, while FPA inhibits this induction. The EAR-lacking isoform of ERF4 acts as a transcriptional activator in vivo and suppresses the flg22-dependent reactive oxygen species burst. We propose that FPA controls use of proximal polyadenylation sites of ERF4, which quantitatively limit the defence response output.
    Original languageEnglish
    Article number2866
    Number of pages10
    JournalScientific Reports
    Volume3
    DOIs
    Publication statusPublished - 9 Oct 2013

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    Polyadenylation
    RNA-Binding Proteins
    Transcription Factors
    Protein Isoforms
    Plant RNA
    Pseudomonas syringae
    Flagellin
    Plant Proteins
    Messenger RNA
    Microarray Analysis
    Arabidopsis
    Reactive Oxygen Species
    Peptides
    ethylene

    Cite this

    Lyons, Rebecca ; Iwase, Akira ; Gänsewig, Thomas ; Sherstnev, Alexander ; Duc, Céline ; Barton, Geoffrey J. ; Hanada, Kousuke ; Higuchi-Takeuchi, Mieko ; Matsui, Minami ; Sugimoto, Keiko ; Kazan, Kemal ; Simpson, Gordon G. ; Shirasu, Ken. / The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation. In: Scientific Reports. 2013 ; Vol. 3.
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    abstract = "RNA-binding proteins (RBPs) play an important role in plant host-microbe interactions. In this study, we show that the plant RBP known as FPA, which regulates 3'-end mRNA polyadenylation, negatively regulates basal resistance to bacterial pathogen Pseudomonas syringae in Arabidopsis. A custom microarray analysis reveals that flg22, a peptide derived from bacterial flagellins, induces expression of alternatively polyadenylated isoforms of mRNA encoding the defence-related transcriptional repressor ETHYLENE RESPONSE FACTOR 4 (ERF4), which is regulated by FPA. Flg22 induces expression of a novel isoform of ERF4 that lacks the ERF-associated amphiphilic repression (EAR) motif, while FPA inhibits this induction. The EAR-lacking isoform of ERF4 acts as a transcriptional activator in vivo and suppresses the flg22-dependent reactive oxygen species burst. We propose that FPA controls use of proximal polyadenylation sites of ERF4, which quantitatively limit the defence response output.",
    author = "Rebecca Lyons and Akira Iwase and Thomas G{\"a}nsewig and Alexander Sherstnev and C{\'e}line Duc and Barton, {Geoffrey J.} and Kousuke Hanada and Mieko Higuchi-Takeuchi and Minami Matsui and Keiko Sugimoto and Kemal Kazan and Simpson, {Gordon G.} and Ken Shirasu",
    year = "2013",
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    Lyons, R, Iwase, A, Gänsewig, T, Sherstnev, A, Duc, C, Barton, GJ, Hanada, K, Higuchi-Takeuchi, M, Matsui, M, Sugimoto, K, Kazan, K, Simpson, GG & Shirasu, K 2013, 'The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation', Scientific Reports, vol. 3, 2866. https://doi.org/10.1038/srep02866

    The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation. / Lyons, Rebecca; Iwase, Akira; Gänsewig, Thomas; Sherstnev, Alexander; Duc, Céline; Barton, Geoffrey J.; Hanada, Kousuke; Higuchi-Takeuchi, Mieko; Matsui, Minami; Sugimoto, Keiko; Kazan, Kemal; Simpson, Gordon G.; Shirasu, Ken.

    In: Scientific Reports, Vol. 3, 2866, 09.10.2013.

    Research output: Contribution to journalArticle

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    AU - Hanada, Kousuke

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    AU - Matsui, Minami

    AU - Sugimoto, Keiko

    AU - Kazan, Kemal

    AU - Simpson, Gordon G.

    AU - Shirasu, Ken

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