TY - JOUR
T1 - The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation
AU - Caretti, Giuseppina
AU - Schiltz, Louis R.
AU - Dilworth, F. Jeffrey
AU - Di Padova, Monica
AU - Zhao, Po
AU - Ogryzko, Vasily
AU - Fuller-Pace, Frances V.
AU - Hoffman, Eric P.
AU - Tapscott, Stephen J.
AU - Sartorelli, Vittorio
N1 - dc.publisher: Elsevier (Cell Press)
dc.description.sponsorship: Intramural Research Program of the National Institute of Arthritis, Musculoskeletal, and Skin Diseases of the National Institutes of Health
PY - 2006/10
Y1 - 2006/10
N2 - MyoD regulates skeletal myogenesis. Since proteins associated with MyoD exert regulatory functions, their identification is expected to contribute important insights into the mechanisms governing gene expression in skeletal muscle. We have found that the RNA helicases p68/p72 are MyoD-associated proteins and that the noncoding RNA SRA also immunoprecipitates with MyoD. In vitro and in vivo experiments indicated that both p68/p72 and SRA are coactivators of MyoD. RNA interference toward either p68/p72 or SRA prevented proper activation of muscle gene expression and cell differentiation. Unexpectedly, reducing the levels of p68/p72 proteins impaired recruitment of the TATA binding protein TBP; RNA polymerase II; and the catalytic subunit of the ATPase SWI/SNF complex, Brg-1, and hindered chromatin remodeling. These findings reveal that p68/p72 play a critical role in promoting the assembly of proteins required for the formation of the transcription initiation complex and chromatin remodeling.
AB - MyoD regulates skeletal myogenesis. Since proteins associated with MyoD exert regulatory functions, their identification is expected to contribute important insights into the mechanisms governing gene expression in skeletal muscle. We have found that the RNA helicases p68/p72 are MyoD-associated proteins and that the noncoding RNA SRA also immunoprecipitates with MyoD. In vitro and in vivo experiments indicated that both p68/p72 and SRA are coactivators of MyoD. RNA interference toward either p68/p72 or SRA prevented proper activation of muscle gene expression and cell differentiation. Unexpectedly, reducing the levels of p68/p72 proteins impaired recruitment of the TATA binding protein TBP; RNA polymerase II; and the catalytic subunit of the ATPase SWI/SNF complex, Brg-1, and hindered chromatin remodeling. These findings reveal that p68/p72 play a critical role in promoting the assembly of proteins required for the formation of the transcription initiation complex and chromatin remodeling.
KW - Cell differentiation physiology
KW - Muscle, skeletal physiology
KW - MyoD protein metabolism
KW - RNA helicases metabolism
KW - RNA untranslated metabolism
U2 - 10.1016/j.devcel.2006.08.003
DO - 10.1016/j.devcel.2006.08.003
M3 - Article
C2 - 17011493
SN - 1534-5807
VL - 11
SP - 547
EP - 560
JO - Developmental Cell
JF - Developmental Cell
IS - 4
ER -