The role of glycolipid C in the GPI biosynthetic pathway in Trypanosoma brucei bloodstream forms

M. L. Güther, W. J. Masterson, M. A. Ferguson

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    The glycosylphosphatidylinositol (GPI) biosynthetic pathway in Trypanosoma brucei bloodstream forms includes the formation of glycolipid C. This molecule is the inositol-acylated form of the GPI anchor precursor, glycolipid A. There is no evidence for the transfer of glycolipid C to protein in vivo and the role of glycolipid C is unclear. In this paper we show that glycolipid C is not synthesised in the presence of phenylmethylsulphonyl fluoride (PMSF) and that glycolipid C is not an obligatory intermediate on the pathway to the formation of glycolipid A. Using pulse-chase experiments we show that glycolipid A and glycolipid C are in a dynamic equilibrium and we suggest that only the forward reaction (glycolipid A conversion to glycolipid C) is inhibited by PMSF.
    Original languageEnglish
    Pages (from-to)121-126
    Number of pages6
    JournalBrazilian Journal of Medical and Biological Research
    Volume27
    Issue number2
    Publication statusPublished - 1994

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