The glycosylphosphatidylinositol (GPI) biosynthetic pathway in Trypanosoma brucei bloodstream forms includes the formation of glycolipid C. This molecule is the inositol-acylated form of the GPI anchor precursor, glycolipid A. There is no evidence for the transfer of glycolipid C to protein in vivo and the role of glycolipid C is unclear. In this paper we show that glycolipid C is not synthesised in the presence of phenylmethylsulphonyl fluoride (PMSF) and that glycolipid C is not an obligatory intermediate on the pathway to the formation of glycolipid A. Using pulse-chase experiments we show that glycolipid A and glycolipid C are in a dynamic equilibrium and we suggest that only the forward reaction (glycolipid A conversion to glycolipid C) is inhibited by PMSF.
|Number of pages||6|
|Journal||Brazilian Journal of Medical and Biological Research|
|Publication status||Published - 1994|
Güther, M. L., Masterson, W. J., & Ferguson, M. A. (1994). The role of glycolipid C in the GPI biosynthetic pathway in Trypanosoma brucei bloodstream forms. Brazilian Journal of Medical and Biological Research, 27(2), 121-126.