The role of PGE2 in the sensitization of mechanoreceptors in normal and inflamed ankle joints of the rat

B. D. Grubb, G. J. Birrell, D. S. McQueen, A. Iggo

    Research output: Contribution to journalArticle

    87 Citations (Scopus)

    Abstract

    The role of PGE2 in the sensitization of highthreshold tarsal joint mechanoreceptors (putative nociceptors) has been investigated in 11 arthritic and 16 normal rats. Injections of a low dose of Freund's complete adjuvant at multiple sites into the tissues surrounding the ankle joint induced a chronic unilateral monoarthritis in the injected limb. Measurements of both spontaneous activity and responses of tarsal joint mechanoreceptors to repeated graded mechanical stimuli were made. All of the mechanoreceptors examined had afferent fibres with conduction velocities in the C or A-δ range. Using this new model of joint inflammation we have shown that lysine acetylsalicylate reduces the mechanical sensitivity of these joint mechanoreceptors and reduces the spontaneous activity in afferent nerve fibres. Prostaglandin E2 is unable to restore either the spontaneous activity in the afferent axon or the mechanical sensitivity of tarsal joint mechanoreceptors after lysine acetylsalicylate in the arthritic rat. Similarly, PGE2 does not sensitize or excite tarsal joint mechanoreceptors in the normal rat. In the normal rat, however, PGE2 potentiates the excitatory action of bradykinin and enhances the sensitizing effect of bradykinin on the responses of joint mechanoreceptors to mechanical stimulation when both substances are injected simultaneously. These results indicate that PGE2 is not important in the sensitization of these joint mechanoreceptors in this model of chronic joint inflammation but that in other circumstances PGE2 may be able to contribute to a sensitization of joint mechanoreceptors by enhancing the action of bradykinin.

    Original languageEnglish
    Pages (from-to)383-392
    Number of pages10
    JournalExperimental Brain Research
    Volume84
    Issue number2
    DOIs
    Publication statusPublished - Apr 1991

    Fingerprint

    Mechanoreceptors
    Ankle Joint
    Dinoprostone
    Tarsal Joints
    Joints
    Bradykinin
    Arthritis
    Inflammation
    Nociceptors
    Freund's Adjuvant
    Nerve Fibers
    Axons
    Extremities
    Injections

    Keywords

    • Arthritic rat joints
    • Bradykinin
    • Chronic inflammation
    • Joint capsule mechanoreceptors
    • PGE2
    • Rat

    Cite this

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    abstract = "The role of PGE2 in the sensitization of highthreshold tarsal joint mechanoreceptors (putative nociceptors) has been investigated in 11 arthritic and 16 normal rats. Injections of a low dose of Freund's complete adjuvant at multiple sites into the tissues surrounding the ankle joint induced a chronic unilateral monoarthritis in the injected limb. Measurements of both spontaneous activity and responses of tarsal joint mechanoreceptors to repeated graded mechanical stimuli were made. All of the mechanoreceptors examined had afferent fibres with conduction velocities in the C or A-δ range. Using this new model of joint inflammation we have shown that lysine acetylsalicylate reduces the mechanical sensitivity of these joint mechanoreceptors and reduces the spontaneous activity in afferent nerve fibres. Prostaglandin E2 is unable to restore either the spontaneous activity in the afferent axon or the mechanical sensitivity of tarsal joint mechanoreceptors after lysine acetylsalicylate in the arthritic rat. Similarly, PGE2 does not sensitize or excite tarsal joint mechanoreceptors in the normal rat. In the normal rat, however, PGE2 potentiates the excitatory action of bradykinin and enhances the sensitizing effect of bradykinin on the responses of joint mechanoreceptors to mechanical stimulation when both substances are injected simultaneously. These results indicate that PGE2 is not important in the sensitization of these joint mechanoreceptors in this model of chronic joint inflammation but that in other circumstances PGE2 may be able to contribute to a sensitization of joint mechanoreceptors by enhancing the action of bradykinin.",
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    The role of PGE2 in the sensitization of mechanoreceptors in normal and inflamed ankle joints of the rat. / Grubb, B. D.; Birrell, G. J.; McQueen, D. S.; Iggo, A.

    In: Experimental Brain Research, Vol. 84, No. 2, 04.1991, p. 383-392.

    Research output: Contribution to journalArticle

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