The role of trehalose in dehydration resistance of Saccharomyces cerevisiae

G. M. Gadd, K. Chalmers, R. H. Reed

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    140 Citations (Scopus)

    Abstract

    High levels of intracellular trehalose in stationary-phase cells of Saccharomyces cerevisiae or cells incubated in the absence of a nitrogen source were found to increase the resistance of the cells to dehydration. Exponential-phase cells showed negligible dehydration resistance. When stationary-phase cells were inoculated into fresh medium, trehalose was rapidly broken down, and this was correlated with a rapid loss of dehydration resistance. It appeared that a minimum internal concentration of 120 mM trehalose was required before there was a significant increase in dehydration resistance. Exogenous trehalose increased the dehydration resistance of S. cerevisiae: this effect was most marked for stationary-phase cells, where almost 100% survival was obtained at trehalose concentrations of 500 mM and above while maximum survival for exponential cells was less than 10%, even at 1000 mM external trehalose.

    Original languageEnglish
    Pages (from-to)249-254
    Number of pages6
    JournalFEMS Microbiology Letters
    Volume48
    Issue number1-2
    DOIs
    Publication statusPublished - 1 Dec 1987

    Keywords

    • Dehydration
    • Saccharomyces cerevisiae
    • Survival
    • Trehalose

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