Abstract
High levels of intracellular trehalose in stationary-phase cells of Saccharomyces cerevisiae or cells incubated in the absence of a nitrogen source were found to increase the resistance of the cells to dehydration. Exponential-phase cells showed negligible dehydration resistance. When stationary-phase cells were inoculated into fresh medium, trehalose was rapidly broken down, and this was correlated with a rapid loss of dehydration resistance. It appeared that a minimum internal concentration of 120 mM trehalose was required before there was a significant increase in dehydration resistance. Exogenous trehalose increased the dehydration resistance of S. cerevisiae: this effect was most marked for stationary-phase cells, where almost 100% survival was obtained at trehalose concentrations of 500 mM and above while maximum survival for exponential cells was less than 10%, even at 1000 mM external trehalose.
| Original language | English |
|---|---|
| Pages (from-to) | 249-254 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 48 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1 Dec 1987 |
Keywords
- Dehydration
- Saccharomyces cerevisiae
- Survival
- Trehalose
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics