The sequential effects of human cervical mucus, oviductal fluid, and follicular fluid on sperm function

TY - JOUR

T1 - The sequential effects of human cervical mucus, oviductal fluid, and follicular fluid on sperm function

AU - Zhu, Jianjun

AU - Barratt, Christopher L. R.

AU - Lippes, Jack

AU - Pacey, Allan A.

AU - Cooke, Ian D.

PY - 1994/6

Y1 - 1994/6

N2 - Objective: To compare the sequential effects of human cervical mucus (CM), oviductal fluid, and follicular fluid (FF) on sperm motility, hyperactivation, and the acrosome reaction during 9 hours of incubation in vitro.Design: Spermatozoa from a fertile donor were allowed to penetrate human CM in vitro and then were recovered and incubated in either 30% human oviductal fluid or a simple salt solution for 3 hours before challenge with 20% FF. Thus, the sequential effects of the fluids collected from the female reproductive tract on sperm hyperactivation and acrosome reaction were examined.Setting: The donor insemination program at the University Clinic within the Jessop Hospital for Women, Sheffield, United Kingdom.Patients: All donors used in this study were involved in the donor insemination program.Main outcome measures: Sperm motility, hyperactivation, and percentage of acrosome-reacted spermatozoa were measured.Results: This study used an in vitro technique to mimic sperm transport in vivo. Spermatozoa sequentially incubated with female reproductive tract secretions in vitro showed a maintenance in the level of FF-induced hyperactivated motility and a decreased response to the FF-induced acrosome reaction when compared with sperm challenged with FF in the absence of prior incubation with human oviductal fluid.Conclusion: It is suggested that human oviductal fluid may serve to delay the FF-induced acrosome reaction.

AB - Objective: To compare the sequential effects of human cervical mucus (CM), oviductal fluid, and follicular fluid (FF) on sperm motility, hyperactivation, and the acrosome reaction during 9 hours of incubation in vitro.Design: Spermatozoa from a fertile donor were allowed to penetrate human CM in vitro and then were recovered and incubated in either 30% human oviductal fluid or a simple salt solution for 3 hours before challenge with 20% FF. Thus, the sequential effects of the fluids collected from the female reproductive tract on sperm hyperactivation and acrosome reaction were examined.Setting: The donor insemination program at the University Clinic within the Jessop Hospital for Women, Sheffield, United Kingdom.Patients: All donors used in this study were involved in the donor insemination program.Main outcome measures: Sperm motility, hyperactivation, and percentage of acrosome-reacted spermatozoa were measured.Results: This study used an in vitro technique to mimic sperm transport in vivo. Spermatozoa sequentially incubated with female reproductive tract secretions in vitro showed a maintenance in the level of FF-induced hyperactivated motility and a decreased response to the FF-induced acrosome reaction when compared with sperm challenged with FF in the absence of prior incubation with human oviductal fluid.Conclusion: It is suggested that human oviductal fluid may serve to delay the FF-induced acrosome reaction.

KW - Acrosome/drug effects

KW - Adult

KW - Body Fluids/chemistry

KW - Cervix Mucus/physiology

KW - Fallopian Tubes/chemistry

KW - Female

KW - Follicular Fluid/physiology

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Male

KW - Progesterone/analysis

KW - Sperm Motility/drug effects

KW - Sperm Transport/physiology

KW - Spermatozoa/cytology

U2 - 10.1016/S0015-0282(16)56768-5

DO - 10.1016/S0015-0282(16)56768-5

M3 - Article

C2 - 8194629

SN - 0015-0282

VL - 61

SP - 1129

EP - 1135

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 6

ER -