The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana

Katarzyna Dorota Raczynska; Agata Stepien; Daniel Kierzkowski; Malgorzata Kalak; Mateusz Bajczyk; Jim McNicol; Craig G. Simpson; Zofia Szweykowska-Kulinska; John W. S. Brown; Artur Jarmolowski

doi:10.1093/nar/gkt894

The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana

Katarzyna Dorota Raczynska, Agata Stepien, Daniel Kierzkowski, Malgorzata Kalak, Mateusz Bajczyk, Jim McNicol, Craig G. Simpson, Zofia Szweykowska-Kulinska (Lead / Corresponding author), John W. S. Brown (Lead / Corresponding author), Artur Jarmolowski (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

277 Downloads (Pure)

Abstract

How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcriptase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 5' splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not correspond to the changes observed in the se-1 mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1 and DCL1, and is similar to the regulation of AS in which CBC is involved.

Original language	English
Pages (from-to)	1224-1244
Number of pages	21
Journal	Nucleic Acids Research
Volume	42
Issue number	2
Early online date	16 Oct 2013
DOIs	https://doi.org/10.1093/nar/gkt894
Publication status	Published - 1 Jan 2014

Access to Document

10.1093/nar/gkt894Licence: CC BY

Final Published VersionFinal published version, 835 KBLicence: CC BY

Brown, John
- DArcy Thompson Unit - Honorary Professor (PhD Supervisor) of Molecular Plant Sciences
Person: Honorary

Cite this

@article{1b299b227ea54fd09c1a03ee22a5ec2b,

title = "The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana",

abstract = "How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcriptase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 5' splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not correspond to the changes observed in the se-1 mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1 and DCL1, and is similar to the regulation of AS in which CBC is involved.",

author = "Raczynska, {Katarzyna Dorota} and Agata Stepien and Daniel Kierzkowski and Malgorzata Kalak and Mateusz Bajczyk and Jim McNicol and Simpson, {Craig G.} and Zofia Szweykowska-Kulinska and Brown, {John W. S.} and Artur Jarmolowski",

year = "2014",

month = jan,

day = "1",

doi = "10.1093/nar/gkt894",

language = "English",

volume = "42",

pages = "1224--1244",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "2",

}

TY - JOUR

T1 - The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana

AU - Raczynska, Katarzyna Dorota

AU - Stepien, Agata

AU - Kierzkowski, Daniel

AU - Kalak, Malgorzata

AU - Bajczyk, Mateusz

AU - McNicol, Jim

AU - Simpson, Craig G.

AU - Szweykowska-Kulinska, Zofia

AU - Brown, John W. S.

AU - Jarmolowski, Artur

PY - 2014/1/1

Y1 - 2014/1/1

N2 - How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcriptase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 5' splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not correspond to the changes observed in the se-1 mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1 and DCL1, and is similar to the regulation of AS in which CBC is involved.

AB - How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcriptase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 5' splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not correspond to the changes observed in the se-1 mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1 and DCL1, and is similar to the regulation of AS in which CBC is involved.

U2 - 10.1093/nar/gkt894

DO - 10.1093/nar/gkt894

M3 - Article

C2 - 24137006

SN - 0305-1048

VL - 42

SP - 1224

EP - 1244

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 2

ER -

The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana

Abstract

Access to Document

Fingerprint

Profiles

Brown, John

Cite this