The structure and folding of branched RNA analyzed by fluorescence resonance energy transfer

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    13 Citations (Scopus)

    Abstract

    Fluorescence resonance energy transfer (FRET) is a spectroscopic means of obtaining distance information over a range up to similar to 80 angstrom in solution. It is based on the dipolar coupling between the electronic transition moments of a donor and acceptor fluorophore attached at known positions on the RNA species of interest. It can be applied in ensembles of molecules, either by steady-state fluorescence or by lifetime measurements, but it is also very appropriate for single-molecule studies. In addition to the provision of distance information, recent studies have emphasized the orientation dependence of energy transfer.

    Original languageEnglish
    Title of host publicationBiophysical, chemical, and functional probes of RNA structure, interactions and folding
    Subtitle of host publicationPart B
    EditorsDaniel Herschlag
    Place of PublicationSan Diego
    PublisherAcademic Press
    Pages159-187
    Number of pages29
    ISBN (Print)9780123809223
    DOIs
    Publication statusPublished - 2009

    Publication series

    NameMethods in Enzymology
    PublisherAcademic Press
    Volume469

    Keywords

    • SINGLE-MOLECULE FRET
    • DOUBLE-STRANDED DNA
    • KINK-TURN MOTIF
    • HAIRPIN RIBOZYME
    • HAMMERHEAD RIBOZYME
    • TERTIARY STRUCTURE
    • SECONDARY STRUCTURE
    • GLOBAL STRUCTURE
    • 4-WAY JUNCTION
    • NUCLEIC-ACIDS

    Cite this

    Lilley, D. M. J. (2009). The structure and folding of branched RNA analyzed by fluorescence resonance energy transfer. In D. Herschlag (Ed.), Biophysical, chemical, and functional probes of RNA structure, interactions and folding : Part B (pp. 159-187). (Methods in Enzymology; Vol. 469). Academic Press. https://doi.org/10.1016/S0076-6879(09)69008-X