Projects per year
Abstract
We have determined high resolution crystal structures of the ykkC guanidine III riboswitch from Thermobifida fusca. The riboswitch forms a classic H-type pseudoknot, in which a connecting strand locates in the major groove of one duplex to form a triple helix. This is continuous with a central core of conserved nucleotides forming a left handed helical ramp of internucleotide interactions that generates the guanidinium cation binding site. The ligand is hydrogen bonded to O6 and N7 of two guanine nucleobases, and to the 2'OH of a cytidine, the nucleobase of which stacks with the guanidinium in a π-cation interaction. The binding pocket has a side opening through which a small side chain can pass, and we have determined structures with bound methylguanidine, aminoguanidine, ethylguanidine and agmatine. Comparison of this structure with those of the guanidine I and II riboswitches reveals common aspects and contrasts.
Original language | English |
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Pages (from-to) | 1407-1415.e2 |
Number of pages | 12 |
Journal | Cell Chemical Biology |
Volume | 24 |
Issue number | 11 |
Early online date | 5 Oct 2017 |
DOIs | |
Publication status | Published - 16 Nov 2017 |
Keywords
- Gene regulation
- RNA structure
- X-ray crystallography
- riboregulation
Fingerprint
Dive into the research topics of 'The structure of the Guanidine-III Riboswitch'. Together they form a unique fingerprint.Projects
- 2 Finished
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Dynamics of Eukaryotic Junction-Resolving Enzyme GEN1 - DNA Junction Interactions
Lilley, D. (Investigator)
Biotechnology and Biological Sciences Research Council
1/10/16 → 30/09/19
Project: Research
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Fluorescence Resonance Energy Transfer as a Rich Source of Orientational Information in Nucleic Acid Structure
Lilley, D. (Investigator)
Engineering and Physical Sciences Research Council
1/09/12 → 30/06/16
Project: Research
Profiles
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Lilley, David
- Molecular Cell and Developmental Biology - Professor of Molecular Biology
Person: Academic