The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4

Triantafyllos Gkikopoulos, Vijender Singh, Kyle Tsui, Salma Awad, Matthew J. Renshaw, Pieta Scholfield, Geoffrey J. Barton, Corey Nislow, Tomoyuki U. Tanaka, Tom Owen-Hughes (Lead / Corresponding author)

Research output: Contribution to journalArticle

33 Citations (Scopus)
65 Downloads (Pure)

Abstract

In order to gain insight into the function of the Saccharomyces cerevisiae SWI/SNF complex, we have identified DNA sequences to which it is bound genomewide. One surprising observation is that the complex is enriched at the centromeres of each chromosome. Deletion of the gene encoding the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites on chromosome arms. Cultures of snf2 Delta yeast were found to progress through mitosis slowly. This was dependent on the mitotic checkpoint protein Mad2. In the absence of Mad2, defects in chromosome segregation were observed. In the absence of Snf2, chromatin organisation at centromeres is less distinct. In particular, hypersensitive sites flanking the Cse4 containing nucleosomes are less pronounced. Furthermore, SWI/SNF complex was found to be especially effective in the dissociation of Cse4 containing chromatin in vitro. This suggests a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites. The EMBO Journal ( \2011) 30, 1919-1927. doi:10.1038/emboj.2011.112; Published online 19 April 2011

Original languageEnglish
Pages (from-to)1919-1927
Number of pages9
JournalEMBO Journal
Volume30
Issue number10
Early online date19 Apr 2011
DOIs
Publication statusPublished - 18 May 2011

Keywords

  • centromere
  • chromatin
  • Cse4
  • nucleosome
  • SWI/SNF
  • CHROMATIN-REMODELING COMPLEX
  • E3 UBIQUITIN LIGASE
  • SACCHAROMYCES-CEREVISIAE
  • BUDDING YEAST
  • H3 VARIANT
  • TRANSCRIPTIONAL ACTIVATION
  • SPINDLE MICROTUBULES
  • NUCLEOSOME OCCUPANCY
  • MITOTIC SPINDLE
  • PROTEIN COMPLEX

Cite this

Gkikopoulos, T., Singh, V., Tsui, K., Awad, S., Renshaw, M. J., Scholfield, P., ... Owen-Hughes, T. (2011). The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4. EMBO Journal, 30(10), 1919-1927. https://doi.org/10.1038/emboj.2011.112
Gkikopoulos, Triantafyllos ; Singh, Vijender ; Tsui, Kyle ; Awad, Salma ; Renshaw, Matthew J. ; Scholfield, Pieta ; Barton, Geoffrey J. ; Nislow, Corey ; Tanaka, Tomoyuki U. ; Owen-Hughes, Tom. / The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4. In: EMBO Journal. 2011 ; Vol. 30, No. 10. pp. 1919-1927.
@article{9fc6f454205a4a7b87c8725ec826171d,
title = "The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4",
abstract = "In order to gain insight into the function of the Saccharomyces cerevisiae SWI/SNF complex, we have identified DNA sequences to which it is bound genomewide. One surprising observation is that the complex is enriched at the centromeres of each chromosome. Deletion of the gene encoding the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites on chromosome arms. Cultures of snf2 Delta yeast were found to progress through mitosis slowly. This was dependent on the mitotic checkpoint protein Mad2. In the absence of Mad2, defects in chromosome segregation were observed. In the absence of Snf2, chromatin organisation at centromeres is less distinct. In particular, hypersensitive sites flanking the Cse4 containing nucleosomes are less pronounced. Furthermore, SWI/SNF complex was found to be especially effective in the dissociation of Cse4 containing chromatin in vitro. This suggests a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites. The EMBO Journal ( \2011) 30, 1919-1927. doi:10.1038/emboj.2011.112; Published online 19 April 2011",
keywords = "centromere, chromatin, Cse4, nucleosome, SWI/SNF, CHROMATIN-REMODELING COMPLEX, E3 UBIQUITIN LIGASE, SACCHAROMYCES-CEREVISIAE, BUDDING YEAST, H3 VARIANT, TRANSCRIPTIONAL ACTIVATION, SPINDLE MICROTUBULES, NUCLEOSOME OCCUPANCY, MITOTIC SPINDLE, PROTEIN COMPLEX",
author = "Triantafyllos Gkikopoulos and Vijender Singh and Kyle Tsui and Salma Awad and Renshaw, {Matthew J.} and Pieta Scholfield and Barton, {Geoffrey J.} and Corey Nislow and Tanaka, {Tomoyuki U.} and Tom Owen-Hughes",
note = "This work was funded by Wellcome Trust Senior Fellowship 064414 (TG, VS, TOH). Work in CN's laboratory is supported by the CFI and CIHR (86705).",
year = "2011",
month = "5",
day = "18",
doi = "10.1038/emboj.2011.112",
language = "English",
volume = "30",
pages = "1919--1927",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "EMBO Press",
number = "10",

}

Gkikopoulos, T, Singh, V, Tsui, K, Awad, S, Renshaw, MJ, Scholfield, P, Barton, GJ, Nislow, C, Tanaka, TU & Owen-Hughes, T 2011, 'The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4', EMBO Journal, vol. 30, no. 10, pp. 1919-1927. https://doi.org/10.1038/emboj.2011.112

The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4. / Gkikopoulos, Triantafyllos; Singh, Vijender; Tsui, Kyle; Awad, Salma; Renshaw, Matthew J.; Scholfield, Pieta; Barton, Geoffrey J.; Nislow, Corey; Tanaka, Tomoyuki U.; Owen-Hughes, Tom (Lead / Corresponding author).

In: EMBO Journal, Vol. 30, No. 10, 18.05.2011, p. 1919-1927.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4

AU - Gkikopoulos, Triantafyllos

AU - Singh, Vijender

AU - Tsui, Kyle

AU - Awad, Salma

AU - Renshaw, Matthew J.

AU - Scholfield, Pieta

AU - Barton, Geoffrey J.

AU - Nislow, Corey

AU - Tanaka, Tomoyuki U.

AU - Owen-Hughes, Tom

N1 - This work was funded by Wellcome Trust Senior Fellowship 064414 (TG, VS, TOH). Work in CN's laboratory is supported by the CFI and CIHR (86705).

PY - 2011/5/18

Y1 - 2011/5/18

N2 - In order to gain insight into the function of the Saccharomyces cerevisiae SWI/SNF complex, we have identified DNA sequences to which it is bound genomewide. One surprising observation is that the complex is enriched at the centromeres of each chromosome. Deletion of the gene encoding the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites on chromosome arms. Cultures of snf2 Delta yeast were found to progress through mitosis slowly. This was dependent on the mitotic checkpoint protein Mad2. In the absence of Mad2, defects in chromosome segregation were observed. In the absence of Snf2, chromatin organisation at centromeres is less distinct. In particular, hypersensitive sites flanking the Cse4 containing nucleosomes are less pronounced. Furthermore, SWI/SNF complex was found to be especially effective in the dissociation of Cse4 containing chromatin in vitro. This suggests a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites. The EMBO Journal ( \2011) 30, 1919-1927. doi:10.1038/emboj.2011.112; Published online 19 April 2011

AB - In order to gain insight into the function of the Saccharomyces cerevisiae SWI/SNF complex, we have identified DNA sequences to which it is bound genomewide. One surprising observation is that the complex is enriched at the centromeres of each chromosome. Deletion of the gene encoding the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites on chromosome arms. Cultures of snf2 Delta yeast were found to progress through mitosis slowly. This was dependent on the mitotic checkpoint protein Mad2. In the absence of Mad2, defects in chromosome segregation were observed. In the absence of Snf2, chromatin organisation at centromeres is less distinct. In particular, hypersensitive sites flanking the Cse4 containing nucleosomes are less pronounced. Furthermore, SWI/SNF complex was found to be especially effective in the dissociation of Cse4 containing chromatin in vitro. This suggests a role for Snf2 in the maintenance of point centromeres involving the removal of Cse4 from ectopic sites. The EMBO Journal ( \2011) 30, 1919-1927. doi:10.1038/emboj.2011.112; Published online 19 April 2011

KW - centromere

KW - chromatin

KW - Cse4

KW - nucleosome

KW - SWI/SNF

KW - CHROMATIN-REMODELING COMPLEX

KW - E3 UBIQUITIN LIGASE

KW - SACCHAROMYCES-CEREVISIAE

KW - BUDDING YEAST

KW - H3 VARIANT

KW - TRANSCRIPTIONAL ACTIVATION

KW - SPINDLE MICROTUBULES

KW - NUCLEOSOME OCCUPANCY

KW - MITOTIC SPINDLE

KW - PROTEIN COMPLEX

U2 - 10.1038/emboj.2011.112

DO - 10.1038/emboj.2011.112

M3 - Article

VL - 30

SP - 1919

EP - 1927

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 10

ER -

Gkikopoulos T, Singh V, Tsui K, Awad S, Renshaw MJ, Scholfield P et al. The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4. EMBO Journal. 2011 May 18;30(10):1919-1927. https://doi.org/10.1038/emboj.2011.112