The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture

A M Schor, S L Schor, T D Allen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Bovine aortic endothelial cells cultured on collagenous or plastic substrata continuously synthesize and deposit a subendothelial matrix, independently of whether the cells are in the logarithmic or the stationary phase of growth. This subendothelial matrix contains fibrillar and amorphous elements comparable with those observed in the subendothelium in vivo. Deposition of subendothelial matrix on a collagen gel substratum both started earlier and progressed at approximately double the rate than that on denatured collagen. The relative composition of the subendothelial matrix was assessed by sequential incubation with trypsin, elastase and collagenase (Jones et al., 1979). The subendothelial matrix deposited on collagen gels by early confluent cultures and late post-confluent cultures differed in their enzyme sensitivity. These age-related changes in the enzyme sensitivity of the subendothelial matrix were characteristic for each cloned cell population examined. Comparable variations in the composition of the subendothelial matrix were not observed when the cells were cultured on plastic or gelatin-coated dishes; the subendothelial matrix deposited on these two substrata contained considerably more trypsin-sensitive material and less elastase and collagenase-sensitive material than the matrix deposited on native collagen gels. Age-related changes in the enzyme sensitivity of the subendothelial matrix deposited on collagen gels was found to be a function of the time elapsed since confluence and it was not related to the time elapsed since plating or to the number of cells present.

Original languageEnglish
Pages (from-to)677-91
Number of pages15
JournalTissue & cell
Volume16
Issue number5
DOIs
Publication statusPublished - 1984

Fingerprint

Collagen
Endothelial Cells
Cell Culture Techniques
Gels
Pancreatic Elastase
Collagenases
Trypsin
Plastics
Enzymes
Gelatin
Cultured Cells
Cell Count
Growth
Population

Keywords

  • Animals
  • Aorta/ultrastructure
  • Ascorbic Acid/pharmacology
  • Cattle
  • Collagen/biosynthesis
  • Culture Media
  • Endothelium/metabolism
  • Extracellular Matrix/metabolism
  • Microscopy, Electron
  • Pancreatic Elastase
  • Trypsin

Cite this

Schor, A M ; Schor, S L ; Allen, T D. / The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture. In: Tissue & cell. 1984 ; Vol. 16, No. 5. pp. 677-91.
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abstract = "Bovine aortic endothelial cells cultured on collagenous or plastic substrata continuously synthesize and deposit a subendothelial matrix, independently of whether the cells are in the logarithmic or the stationary phase of growth. This subendothelial matrix contains fibrillar and amorphous elements comparable with those observed in the subendothelium in vivo. Deposition of subendothelial matrix on a collagen gel substratum both started earlier and progressed at approximately double the rate than that on denatured collagen. The relative composition of the subendothelial matrix was assessed by sequential incubation with trypsin, elastase and collagenase (Jones et al., 1979). The subendothelial matrix deposited on collagen gels by early confluent cultures and late post-confluent cultures differed in their enzyme sensitivity. These age-related changes in the enzyme sensitivity of the subendothelial matrix were characteristic for each cloned cell population examined. Comparable variations in the composition of the subendothelial matrix were not observed when the cells were cultured on plastic or gelatin-coated dishes; the subendothelial matrix deposited on these two substrata contained considerably more trypsin-sensitive material and less elastase and collagenase-sensitive material than the matrix deposited on native collagen gels. Age-related changes in the enzyme sensitivity of the subendothelial matrix deposited on collagen gels was found to be a function of the time elapsed since confluence and it was not related to the time elapsed since plating or to the number of cells present.",
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Schor, AM, Schor, SL & Allen, TD 1984, 'The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture', Tissue & cell, vol. 16, no. 5, pp. 677-91. https://doi.org/10.1016/0040-8166(84)90002-8

The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture. / Schor, A M; Schor, S L; Allen, T D.

In: Tissue & cell, Vol. 16, No. 5, 1984, p. 677-91.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The synthesis of subendothelial matrix by bovine aortic endothelial cells in culture

AU - Schor, A M

AU - Schor, S L

AU - Allen, T D

PY - 1984

Y1 - 1984

N2 - Bovine aortic endothelial cells cultured on collagenous or plastic substrata continuously synthesize and deposit a subendothelial matrix, independently of whether the cells are in the logarithmic or the stationary phase of growth. This subendothelial matrix contains fibrillar and amorphous elements comparable with those observed in the subendothelium in vivo. Deposition of subendothelial matrix on a collagen gel substratum both started earlier and progressed at approximately double the rate than that on denatured collagen. The relative composition of the subendothelial matrix was assessed by sequential incubation with trypsin, elastase and collagenase (Jones et al., 1979). The subendothelial matrix deposited on collagen gels by early confluent cultures and late post-confluent cultures differed in their enzyme sensitivity. These age-related changes in the enzyme sensitivity of the subendothelial matrix were characteristic for each cloned cell population examined. Comparable variations in the composition of the subendothelial matrix were not observed when the cells were cultured on plastic or gelatin-coated dishes; the subendothelial matrix deposited on these two substrata contained considerably more trypsin-sensitive material and less elastase and collagenase-sensitive material than the matrix deposited on native collagen gels. Age-related changes in the enzyme sensitivity of the subendothelial matrix deposited on collagen gels was found to be a function of the time elapsed since confluence and it was not related to the time elapsed since plating or to the number of cells present.

AB - Bovine aortic endothelial cells cultured on collagenous or plastic substrata continuously synthesize and deposit a subendothelial matrix, independently of whether the cells are in the logarithmic or the stationary phase of growth. This subendothelial matrix contains fibrillar and amorphous elements comparable with those observed in the subendothelium in vivo. Deposition of subendothelial matrix on a collagen gel substratum both started earlier and progressed at approximately double the rate than that on denatured collagen. The relative composition of the subendothelial matrix was assessed by sequential incubation with trypsin, elastase and collagenase (Jones et al., 1979). The subendothelial matrix deposited on collagen gels by early confluent cultures and late post-confluent cultures differed in their enzyme sensitivity. These age-related changes in the enzyme sensitivity of the subendothelial matrix were characteristic for each cloned cell population examined. Comparable variations in the composition of the subendothelial matrix were not observed when the cells were cultured on plastic or gelatin-coated dishes; the subendothelial matrix deposited on these two substrata contained considerably more trypsin-sensitive material and less elastase and collagenase-sensitive material than the matrix deposited on native collagen gels. Age-related changes in the enzyme sensitivity of the subendothelial matrix deposited on collagen gels was found to be a function of the time elapsed since confluence and it was not related to the time elapsed since plating or to the number of cells present.

KW - Animals

KW - Aorta/ultrastructure

KW - Ascorbic Acid/pharmacology

KW - Cattle

KW - Collagen/biosynthesis

KW - Culture Media

KW - Endothelium/metabolism

KW - Extracellular Matrix/metabolism

KW - Microscopy, Electron

KW - Pancreatic Elastase

KW - Trypsin

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