The U6 small nuclear RNA gene family of potato

F. Guerineau (Lead / Corresponding author), R. Waugh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Using the inverse polymerase chain reaction (IPCR), 19 U6snRNA gene promoters were isolated from the potato genome. Analysis of their nucleotide sequences revealed the existence of two subfamilies. Promoters from class 1 harbour the typical sequence elements required for plant snRNA gene transcription whereas those from class 2 do not have a TATA box. Three promoters were fused to a modified U6snRNA-coding sequence to allow their acitivity to be monitored in tobacco protoplasts. Two of the promoters, one from either class, were found to be active. Comparison of potato U6snRNA gene promoter sequences with those found in other plant species showed various degrees of homology. In addition, the entire nucleotide sequences of seven potato U6snRNA genes and one pseudogene were determined. The overall frequency of nucleotide changes after PCR was found to be 1.15×10-3. The mutations appeared to be clustered in a distinct area and were all A-to-G/T-to-C substitutions.

Original languageEnglish
Pages (from-to)807-818
Number of pages12
JournalPlant Molecular Biology
Volume22
Issue number5
DOIs
Publication statusPublished - 1 Aug 1993

Fingerprint

small nuclear RNA
Solanum tuberosum
promoter regions
potatoes
Genes
Small Nuclear RNA
Plant Genes
Polymerase Chain Reaction
TATA Box
Pseudogenes
genes
Protoplasts
Tobacco
nucleotide sequences
Nucleotides
TATA box
Genome
pseudogenes
Mutation
protoplasts

Keywords

  • inverse PCR
  • multigene family
  • potato
  • promoter
  • U6snRNA

Cite this

Guerineau, F. ; Waugh, R. / The U6 small nuclear RNA gene family of potato. In: Plant Molecular Biology. 1993 ; Vol. 22, No. 5. pp. 807-818.
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The U6 small nuclear RNA gene family of potato. / Guerineau, F. (Lead / Corresponding author); Waugh, R.

In: Plant Molecular Biology, Vol. 22, No. 5, 01.08.1993, p. 807-818.

Research output: Contribution to journalArticle

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