The use of in vitro immunisation, as an adjunct to monoclonal antibody production, may result in the production of hybridomas secreting polyreactive antibodies

Michael J. McMahon, Richard O'Kennedy

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The possibility that immortalisation of in vitro immunised splenocytes may result in hybridomas secreting polyreactive antibodies was investigated. A panel of nine murine hybridomas, secreting IgM(kappa) anti-goat immunoglobulin G (anti-GIgG), was produced by immortalising splenocytes that had been immunised in vitro with GIgG. The ability of the corresponding monoclonal antibodies (Mabs) to bind multiple antigens was investigated using two techniques. First, the affinity constants characterising the interactions of each of the nine Mabs with each of a panel of six antigens were determined. Second, the specific anti-GIgG activities of each hybridoma supernatant and its corresponding affinity-purified IgM fraction were determined and compared. In total, these experiments indicated that eight of the nine hybridomas were polyreactive.
    Original languageEnglish
    Pages (from-to)27-36
    Number of pages10
    JournalJournal of immunological methods
    Volume258
    Issue number1-2
    DOIs
    Publication statusPublished - 1 Dec 2001

    Fingerprint

    Hybridomas
    Antibody Formation
    Immunization
    Monoclonal Antibodies
    Antibodies
    Goats
    Immunoglobulin G
    Antigens
    Immunoglobulin M
    In Vitro Techniques

    Keywords

    • In vitro immunisation
    • Polyreactive
    • Hybridoma

    Cite this

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    abstract = "The possibility that immortalisation of in vitro immunised splenocytes may result in hybridomas secreting polyreactive antibodies was investigated. A panel of nine murine hybridomas, secreting IgM(kappa) anti-goat immunoglobulin G (anti-GIgG), was produced by immortalising splenocytes that had been immunised in vitro with GIgG. The ability of the corresponding monoclonal antibodies (Mabs) to bind multiple antigens was investigated using two techniques. First, the affinity constants characterising the interactions of each of the nine Mabs with each of a panel of six antigens were determined. Second, the specific anti-GIgG activities of each hybridoma supernatant and its corresponding affinity-purified IgM fraction were determined and compared. In total, these experiments indicated that eight of the nine hybridomas were polyreactive.",
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    The use of in vitro immunisation, as an adjunct to monoclonal antibody production, may result in the production of hybridomas secreting polyreactive antibodies. / McMahon, Michael J.; O'Kennedy, Richard.

    In: Journal of immunological methods, Vol. 258, No. 1-2, 01.12.2001, p. 27-36.

    Research output: Contribution to journalArticle

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