The use of the Rx spin label in orientation measurement on proteins, by EPR

M. A. Stevens, J. E. McKay, J. L S Robinson, H. El Mkami, G. M. Smith, D. G. Norman (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

The bipedal spin label Rx is more restricted in its conformation and dynamics than its monopodal counterpart R1. To systematically investigate the utility of the Rx label, we have attempted to comprehensively survey the attachment of Rx to protein secondary structures. We have examined the formation, structure and dynamics of the spin label in relation to the underlying protein in order to determine feasibility and optimum conditions for distance and orientation measurement by pulsed EPR. The labeled proteins have been studied using molecular dynamics, CW EPR, pulsed EPR distance measurement at X-band and orientation measurement at W-band. The utility of different modes and positions of attachment have been compared and contrasted.

Original languageEnglish
Pages (from-to)5799-5806
Number of pages8
JournalPhysical Chemistry Chemical Physics
Volume18
Issue number8
Early online date25 Sep 2015
DOIs
Publication statusPublished - 2016

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Spin Labels
Paramagnetic resonance
proteins
attachment
Proteins
Distance measurement
superhigh frequencies
Conformations
Molecular dynamics
Labels
molecular dynamics

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Stevens, M. A. ; McKay, J. E. ; Robinson, J. L S ; El Mkami, H. ; Smith, G. M. ; Norman, D. G. / The use of the Rx spin label in orientation measurement on proteins, by EPR. In: Physical Chemistry Chemical Physics. 2016 ; Vol. 18, No. 8. pp. 5799-5806.
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Stevens, MA, McKay, JE, Robinson, JLS, El Mkami, H, Smith, GM & Norman, DG 2016, 'The use of the Rx spin label in orientation measurement on proteins, by EPR', Physical Chemistry Chemical Physics, vol. 18, no. 8, pp. 5799-5806. https://doi.org/10.1039/c5cp04753f

The use of the Rx spin label in orientation measurement on proteins, by EPR. / Stevens, M. A.; McKay, J. E.; Robinson, J. L S; El Mkami, H.; Smith, G. M.; Norman, D. G. (Lead / Corresponding author).

In: Physical Chemistry Chemical Physics, Vol. 18, No. 8, 2016, p. 5799-5806.

Research output: Contribution to journalArticle

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