The use of the Rx spin label in orientation measurement on proteins, by EPR

M. A. Stevens, J. E. McKay, J. L S Robinson, H. El Mkami, G. M. Smith, D. G. Norman (Lead / Corresponding author)

Research output: Contribution to journalArticle

9 Citations (Scopus)
128 Downloads (Pure)

Abstract

The bipedal spin label Rx is more restricted in its conformation and dynamics than its monopodal counterpart R1. To systematically investigate the utility of the Rx label, we have attempted to comprehensively survey the attachment of Rx to protein secondary structures. We have examined the formation, structure and dynamics of the spin label in relation to the underlying protein in order to determine feasibility and optimum conditions for distance and orientation measurement by pulsed EPR. The labeled proteins have been studied using molecular dynamics, CW EPR, pulsed EPR distance measurement at X-band and orientation measurement at W-band. The utility of different modes and positions of attachment have been compared and contrasted.

Original languageEnglish
Pages (from-to)5799-5806
Number of pages8
JournalPhysical Chemistry Chemical Physics
Volume18
Issue number8
Early online date25 Sep 2015
DOIs
Publication statusPublished - 2016

Fingerprint Dive into the research topics of 'The use of the Rx spin label in orientation measurement on proteins, by EPR'. Together they form a unique fingerprint.

  • Student Theses

    The Application of Pulsed Electron Paramagnetic Resonance to the Investigation of Protein Structure

    Author: Stevens, M., 2018

    Supervisor: Norman, D. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

    File

    Profiles

    No photo of David Norman

    Norman, David

    Person: Academic

    Cite this