Three-photon light-sheet fluorescence microscopy

TY - JOUR

T1 - Three-photon light-sheet fluorescence microscopy

AU - Escobet-Montalbán, Adrià

AU - Gasparoli, Federico M.

AU - Nylk, Jonathan

AU - Liu, Pengfei

AU - Yang, Zhengyi

AU - Dholakia, Kishan

PY - 2018/11/1

Y1 - 2018/11/1

N2 - We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low-photodamage technique for fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime, enhancing its penetration depth. Our present study uses a conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 μm diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams.

AB - We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low-photodamage technique for fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime, enhancing its penetration depth. Our present study uses a conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 μm diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams.

UR - https://www.scopus.com/pages/publications/85056096407

U2 - 10.1364/OL.43.005484

DO - 10.1364/OL.43.005484

M3 - Article

C2 - 30383037

AN - SCOPUS:85056096407

SN - 0146-9592

VL - 43

SP - 5484

EP - 5487

JO - Optics Letters

JF - Optics Letters

IS - 21

ER -